Assessment of exposure to fungi in the heavily contaminated work environment (a solid waste sorting plant) based on the ergosterol analysis.

2.50
Hdl Handle:
http://hdl.handle.net/10146/565712
Title:
Assessment of exposure to fungi in the heavily contaminated work environment (a solid waste sorting plant) based on the ergosterol analysis.
Authors:
Kozajda, Anna Iwona ( 0000-0001-5533-9675 ) ; Jezak, Karolina; Sowiak, Malgorzata; Gutarowska, Beata; Szadkowska-Stanczyk, Irena
Abstract:
This paper reports on the results of the study aimed at application of ergosterol as an quantitative indicator of fungal bioaerosol present in the indoor air in occupational environment heavily contaminated with organic dust as well as its comparison with the culturable method.; The study was conducted in the indoor solid waste sorting plant. Using Andersen impactor adapted to 1 plate at the flow rate of 30 l/min, indoor air was sampled in the workers' breathing zone. Ergosterol was sampled using gelatinous filter (1000 l of air) and then analyzed by means of the spectrophotometric method. Fungi were sampled on malt extract agar (MEA) medium (3 replications: 2 l, 7.5 l, 15 l of air) and analyzed by means of the culturable method. Based on ergosterol analyzes, concentration of fungi was calculated. Results were given as the range assuming min. as 5.1 pg ergosterol/spore and max as 1.7 pg ergosterol/spore.; The average concentrations of ergosterol in a working room (arithmetic mean (AM), standard deviation (SD); minimum-maximum (min.-max)) were, respectively: 2.16, 0.72; 0.85-2.92 μg/m3; fungi calculated based on ergosterol - 424.1×103-1272.4×103, 140.1×103- 420.4×103, 167×103-1716.5×103 CFU/m3, and culturable fungi - 13×103, 9.7×103, 1.9×103-34×103 CFU/m3). It was revealed that concentrations of calculated fungi were even 2 orders of magnitude higher than culturable fungi.; The quantitative assessment of moldiness by means of ergosterol measurement seems to be a reliable indicator for environments heavily contaminated with organic dust, where viable and non-viable fungi are present in high proportions. Based on that result, more restrictive (as compared to a similar assessment carried out by means of the culturable method) hygienic recommendations, especially those related to the use of preventive measures protecting the employees' respiratory tract, should have been undertaken.
Affiliation:
Nofer Institute of Occupational Medicine, Łódź, Poland; Lodz University of Technology, Łódź, Poland
Citation:
Int J Occup Med Environ Health. 2015;28(5):813-21
Journal:
International Journal of Occupational Medicine and Environmental Health
Issue Date:
2015
URI:
http://hdl.handle.net/10146/565712
DOI:
10.13075/ijomeh.1896.00455
PubMed ID:
26224493
Additional Links:
http://ijomeh.eu/Assessment-of-exposure-to-fungi-in-the-heavily-contaminated-work-environment-a-solid-waste-sorting-plant-based-on-the-ergosterol-analysis,58541,0,2.html
Type:
Article
Language:
en
ISSN:
1896-494X
Sponsors:
The project was financed with a grant for statutory activity of the Nofer Institute of Occupational Medicine, Łódź, Poland, from the subsidy No. IMP 3.5/2008
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKozajda, Anna Iwonaen
dc.contributor.authorJezak, Karolinaen
dc.contributor.authorSowiak, Malgorzataen
dc.contributor.authorGutarowska, Beataen
dc.contributor.authorSzadkowska-Stanczyk, Irenaen
dc.date.accessioned2015-08-07T08:01:48Zen
dc.date.available2015-08-07T08:01:48Zen
dc.date.issued2015en
dc.identifier.citationInt J Occup Med Environ Health. 2015;28(5):813-21en
dc.identifier.issn1896-494Xen
dc.identifier.pmid26224493en
dc.identifier.doi10.13075/ijomeh.1896.00455en
dc.identifier.urihttp://hdl.handle.net/10146/565712en
dc.description.abstractThis paper reports on the results of the study aimed at application of ergosterol as an quantitative indicator of fungal bioaerosol present in the indoor air in occupational environment heavily contaminated with organic dust as well as its comparison with the culturable method.en
dc.description.abstractThe study was conducted in the indoor solid waste sorting plant. Using Andersen impactor adapted to 1 plate at the flow rate of 30 l/min, indoor air was sampled in the workers' breathing zone. Ergosterol was sampled using gelatinous filter (1000 l of air) and then analyzed by means of the spectrophotometric method. Fungi were sampled on malt extract agar (MEA) medium (3 replications: 2 l, 7.5 l, 15 l of air) and analyzed by means of the culturable method. Based on ergosterol analyzes, concentration of fungi was calculated. Results were given as the range assuming min. as 5.1 pg ergosterol/spore and max as 1.7 pg ergosterol/spore.en
dc.description.abstractThe average concentrations of ergosterol in a working room (arithmetic mean (AM), standard deviation (SD); minimum-maximum (min.-max)) were, respectively: 2.16, 0.72; 0.85-2.92 μg/m3; fungi calculated based on ergosterol - 424.1×103-1272.4×103, 140.1×103- 420.4×103, 167×103-1716.5×103 CFU/m3, and culturable fungi - 13×103, 9.7×103, 1.9×103-34×103 CFU/m3). It was revealed that concentrations of calculated fungi were even 2 orders of magnitude higher than culturable fungi.en
dc.description.abstractThe quantitative assessment of moldiness by means of ergosterol measurement seems to be a reliable indicator for environments heavily contaminated with organic dust, where viable and non-viable fungi are present in high proportions. Based on that result, more restrictive (as compared to a similar assessment carried out by means of the culturable method) hygienic recommendations, especially those related to the use of preventive measures protecting the employees' respiratory tract, should have been undertaken.en
dc.description.sponsorshipThe project was financed with a grant for statutory activity of the Nofer Institute of Occupational Medicine, Łódź, Poland, from the subsidy No. IMP 3.5/2008en
dc.language.isoenen
dc.relation.urlhttp://ijomeh.eu/Assessment-of-exposure-to-fungi-in-the-heavily-contaminated-work-environment-a-solid-waste-sorting-plant-based-on-the-ergosterol-analysis,58541,0,2.htmlen
dc.rightsArchived with thanks to International journal of occupational medicine and environmental healthen
dc.subjectbioaerosolsen
dc.subjectergosterolen
dc.subjectindoor air pollutionen
dc.subjectoccupational exposureen
dc.subjectsolid waste management planten
dc.subjectsterolsen
dc.titleAssessment of exposure to fungi in the heavily contaminated work environment (a solid waste sorting plant) based on the ergosterol analysis.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicine, Łódź, Poland; Lodz University of Technology, Łódź, Polanden
dc.identifier.journalInternational Journal of Occupational Medicine and Environmental Healthen

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