Cytochrome P450 1B1, a novel chemopreventive target for benzo[a]pyrene-initiated human esophageal cancer.

2.50
Hdl Handle:
http://hdl.handle.net/10146/55913
Title:
Cytochrome P450 1B1, a novel chemopreventive target for benzo[a]pyrene-initiated human esophageal cancer.
Authors:
Wen, Xia; Walle, Thomas
Abstract:
Esophageal cancer is common worldwide, with poor prognosis. Smoking, including exposure to polyaromatic hydrocarbons like benzo[a]pyrene (BaP), is a major risk factor. In human esophageal HET-1A cells, we found that time-dependent BaP-DNA binding was associated with upregulation of CYP1B1, but not CYP1A1, mRNA and protein. The dietary flavonoid 5,7-dimethoxyflavone significantly inhibited BaP-DNA binding and down-regulated BaP-induced CYP1B1 mRNA and protein. 3',4'-Dimethoxyflavone was an even more potent inhibitor of CYP1B1 expression, while resveratrol had no effect. Thus, dietary methoxylated flavones inhibited BaP-induced CYP1B1 transcription in a cell-specific manner and hold promise as chemopreventive agents in esophageal carcinogenesis.
Citation:
Cancer Lett. 2007, 246 (1-2):109-114
Journal:
Cancer letters
Issue Date:
8-Feb-2007
URI:
http://hdl.handle.net/10146/55913
DOI:
10.1016/j.canlet.2006.02.003
PubMed ID:
16530937
Additional Links:
http://www.cancerletters.info/article/S0304-3835(06)00107-8/abstract
Type:
Article
Language:
en
Description:
Dietary modulation of carcinogenesis-related pathwaysDietary item or component studied: 5,7- dimethoxyflavone (5,7-DMF) and 30,40-dimethoxyflavone (30,40-DMF)Pathways studied: BaP- DNA binding by inhibition of CYP1B1/1A1 activity and/or protein expressionStudy type (in vitro, animals, humans): in vitroImpact on pathway (including dose-response): 5,7-DMF: BaP-DNA binding was markedly inhibited and the BaP-induced part of the CYP1B1 mRNA expression (P<0.05)30,40-DMF: showed a more potent inhibition of BaP-induced CYP1B1 mRNA (P<0.05. KEYWORDS CLASSIFICATION: dietary modulation of carcinogenesis-related pathways;mechanisms of carcinogenesis;Research;Smoking;
ISSN:
0304-3835
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorWen, Xia-
dc.contributor.authorWalle, Thomas-
dc.date.accessioned2009-03-17T12:56:30Z-
dc.date.available2009-03-17T12:56:30Z-
dc.date.issued2007-02-08-
dc.identifier.citationCancer Lett. 2007, 246 (1-2):109-114en
dc.identifier.issn0304-3835-
dc.identifier.pmid16530937-
dc.identifier.doi10.1016/j.canlet.2006.02.003-
dc.identifier.urihttp://hdl.handle.net/10146/55913-
dc.descriptionDietary modulation of carcinogenesis-related pathwaysDietary item or component studied: 5,7- dimethoxyflavone (5,7-DMF) and 30,40-dimethoxyflavone (30,40-DMF)Pathways studied: BaP- DNA binding by inhibition of CYP1B1/1A1 activity and/or protein expressionStudy type (in vitro, animals, humans): in vitroImpact on pathway (including dose-response): 5,7-DMF: BaP-DNA binding was markedly inhibited and the BaP-induced part of the CYP1B1 mRNA expression (P<0.05)30,40-DMF: showed a more potent inhibition of BaP-induced CYP1B1 mRNA (P<0.05. KEYWORDS CLASSIFICATION: dietary modulation of carcinogenesis-related pathways;mechanisms of carcinogenesis;Research;Smoking;en
dc.description.abstractEsophageal cancer is common worldwide, with poor prognosis. Smoking, including exposure to polyaromatic hydrocarbons like benzo[a]pyrene (BaP), is a major risk factor. In human esophageal HET-1A cells, we found that time-dependent BaP-DNA binding was associated with upregulation of CYP1B1, but not CYP1A1, mRNA and protein. The dietary flavonoid 5,7-dimethoxyflavone significantly inhibited BaP-DNA binding and down-regulated BaP-induced CYP1B1 mRNA and protein. 3',4'-Dimethoxyflavone was an even more potent inhibitor of CYP1B1 expression, while resveratrol had no effect. Thus, dietary methoxylated flavones inhibited BaP-induced CYP1B1 transcription in a cell-specific manner and hold promise as chemopreventive agents in esophageal carcinogenesis.en
dc.language.isoenen
dc.relation.urlhttp://www.cancerletters.info/article/S0304-3835(06)00107-8/abstracten
dc.subjectEsophageal carcinogenesisen
dc.subjectBenzo[a]pyreneen
dc.subjectCYP1B1en
dc.subjectMethoxylated flavonesen
dc.subject.meshAryl Hydrocarbon Hydroxylases-
dc.subject.meshBenzo(a)pyrene-
dc.subject.meshBlotting, Western-
dc.subject.meshCell Line-
dc.subject.meshCytochrome P-450 CYP1A1-
dc.subject.meshDNA-
dc.subject.meshDNA Adducts-
dc.subject.meshEsophageal Neoplasms-
dc.subject.meshFlavones-
dc.subject.meshFlavonoids-
dc.subject.meshGene Expression Regulation, Enzymologic-
dc.subject.meshHumans-
dc.subject.meshRNA, Messenger-
dc.subject.meshTime Factors-
dc.titleCytochrome P450 1B1, a novel chemopreventive target for benzo[a]pyrene-initiated human esophageal cancer.en
dc.typeArticleen
dc.identifier.journalCancer lettersen
All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.