Biological monitoring and the influence of genetic polymorphism of As3MT and GSTs on distribution of urinary arsenic species in occupational exposure workers.

2.50
Hdl Handle:
http://hdl.handle.net/10146/556831
Title:
Biological monitoring and the influence of genetic polymorphism of As3MT and GSTs on distribution of urinary arsenic species in occupational exposure workers.
Authors:
Janasik, Beata ( 0000-0002-0904-9869 ) ; Reszka, Edyta ( 0000-0003-2153-4864 ) ; Stanislawska, Magdalena; Wieczorek, Edyta; Fendler, Wojciech ( 0000-0002-5083-9168 ) ; Wasowicz, Wojciech ( 0000-0002-2991-9040 )
Abstract:
Purpose To examine the differences in urinary arsenic metabolism patterns in men affected by occupational exposure, we performed a study on 149 participants—workers of a copper mill and 52 healthy controls without occupational exposure. To elucidate the role of genetic factors in arsenic (As) metabolism, we studied the associations of six polymorphisms: As3MT Met287Thr (T>C) in exon 9; As3MT A>G in 5′UTR; As3MT C>G in intron 6; As3MT T>G in intron 1; GSTP1 Ile105Val and GSTO2 T>C. Methods Air samples were collected using individual samplers during work shift. Urine samples were analyzed for total arsenic and arsenic chemical forms (AsIII; AsV, MMA, DMA, AsB) using HPLC–ICP-MS. A specific polymerase chain reaction was done for the amplification of exons and flanking regions of As3MT and GSTs. Results The geometric mean arsenic concentrations in the air were 27.6 ± 4.9 μg/m3. A significant correlation (p < 0.05) was observed between arsenic in air and sum of iAs +MMA and iAs. As3MT (rs3740400) GG homozygotes showed significantly (p < 0.05) higher %iAs (21.8 ± 2.0) in urine than GC+CC heterozygotes (16.0 ± 2.1). A strong association between the gene variants and As species in urine was observed for GSTO2 (rs156697) polymorphism. Conclusions The findings of the study point out that the concentration of iAs or the sum of iAs + MMA in urine can be a reliable biological indicator of occupational exposure to arsenic. This study demonstrates that As3MT and/ or GSTs genotype may influence As metabolism. Nevertheless, further studies investigating genetic polymorphism in occupational conditions are required.
Affiliation:
Nofer Institute of Occupational Medicine, Łódź, Poland; Medical University of Lodz, Łódź, Poland; Medical University of Lodz, Lodz, Poland
Journal:
International Archives of Occupational and Environmental Health International Archives of Occupational and Environmental Health
Issue Date:
2015
URI:
http://hdl.handle.net/10146/556831
Additional Links:
http://link.springer.com/article/10.1007%2Fs00420-014-1009-7
Type:
Article
Language:
en
ISSN:
1432-1246
Sponsors:
Grant for Scientific Research (0170/B/PO1/2010/38) of the Polish Ministry of Science and Higher Education and the internal grant IMP 4.6. from the statutory activity resources of the Nofer Institute of Occupational Medicine in Lodz.
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Full metadata record

DC FieldValue Language
dc.contributor.authorJanasik, Beataen
dc.contributor.authorReszka, Edytaen
dc.contributor.authorStanislawska, Magdalenaen
dc.contributor.authorWieczorek, Edytaen
dc.contributor.authorFendler, Wojciechen
dc.contributor.authorWasowicz, Wojciechen
dc.date.accessioned2015-06-12T09:33:35Zen
dc.date.available2015-06-12T09:33:35Zen
dc.date.issued2015en
dc.identifier.issn1432-1246en
dc.identifier.urihttp://hdl.handle.net/10146/556831en
dc.description.abstractPurpose To examine the differences in urinary arsenic metabolism patterns in men affected by occupational exposure, we performed a study on 149 participants—workers of a copper mill and 52 healthy controls without occupational exposure. To elucidate the role of genetic factors in arsenic (As) metabolism, we studied the associations of six polymorphisms: As3MT Met287Thr (T>C) in exon 9; As3MT A>G in 5′UTR; As3MT C>G in intron 6; As3MT T>G in intron 1; GSTP1 Ile105Val and GSTO2 T>C. Methods Air samples were collected using individual samplers during work shift. Urine samples were analyzed for total arsenic and arsenic chemical forms (AsIII; AsV, MMA, DMA, AsB) using HPLC–ICP-MS. A specific polymerase chain reaction was done for the amplification of exons and flanking regions of As3MT and GSTs. Results The geometric mean arsenic concentrations in the air were 27.6 ± 4.9 μg/m3. A significant correlation (p < 0.05) was observed between arsenic in air and sum of iAs +MMA and iAs. As3MT (rs3740400) GG homozygotes showed significantly (p < 0.05) higher %iAs (21.8 ± 2.0) in urine than GC+CC heterozygotes (16.0 ± 2.1). A strong association between the gene variants and As species in urine was observed for GSTO2 (rs156697) polymorphism. Conclusions The findings of the study point out that the concentration of iAs or the sum of iAs + MMA in urine can be a reliable biological indicator of occupational exposure to arsenic. This study demonstrates that As3MT and/ or GSTs genotype may influence As metabolism. Nevertheless, further studies investigating genetic polymorphism in occupational conditions are required.en
dc.description.sponsorshipGrant for Scientific Research (0170/B/PO1/2010/38) of the Polish Ministry of Science and Higher Education and the internal grant IMP 4.6. from the statutory activity resources of the Nofer Institute of Occupational Medicine in Lodz.en
dc.language.isoenen
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs00420-014-1009-7en
dc.subjectbiological monitoringen
dc.subjectarsenicen
dc.subjectAs3MTen
dc.subjectGSTen
dc.subjectpolymorphismen
dc.subjecthumanen
dc.subjecturineen
dc.titleBiological monitoring and the influence of genetic polymorphism of As3MT and GSTs on distribution of urinary arsenic species in occupational exposure workers.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicine, Łódź, Poland; Medical University of Lodz, Łódź, Poland; Medical University of Lodz, Lodz, Polanden
dc.identifier.journalInternational Archives of Occupational and Environmental Health International Archives of Occupational and Environmental Healthen
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