Genetic variation of GSTM1, GSTT1 and GSTP1 genes in a South Indian population.

2.50
Hdl Handle:
http://hdl.handle.net/10146/54153
Title:
Genetic variation of GSTM1, GSTT1 and GSTP1 genes in a South Indian population.
Authors:
Vettriselvi, V.; Vijayalakshmi, K.; Solomon Fd, Paul; Venkatachalam, P.
Abstract:
The glutathione S transferase (GST) family of enzymes play a vital role in the phase II biotransformation of environmental carcinogens, pollutants, drugs and other xenobiotics. GSTs are polymorphic and the polymorphisms in GST genes have been associated with cancer susceptibility and prognosis. Moreover, distinct ethnic differences have been observed in the type and frequency of GST gene polymorphisms. Hence, the present study was aimed to determine the frequencies of GSTM1, GSTT1 and GSTP1 polymorphisms in 255 healthy random volunteers from South India. The GSTM1 and GSTT1 genotypes were determined by PCR and GSTP1 by PCR-RFLP using peripheral blood DNA.The GSTM1 and GSTT1 null genotype frequencies were found to be 22.4% and 17.6% respectively. The GSTP1 allelic frequency was 0.78 for the Ile allele and 0.22 for the Val allele and the genotype frequency was 58.4% for Ile/Ile, 38.4% for Ile/Val, and 3.1% for Val/Val. Comparison of the frequencies of GST polymorphisms observed in the present study with other Indian and world populations revealed a distinctive nature of the South Indian population with respect to polymorphims at the GST gene loci. A better understanding of carcinogen metabolizing gene distribution should contribute to risk assessment of humans exposed to environmental carcinogens.
Citation:
Asian Pac. J. Cancer Prev., 7 (2):325-328
Journal:
Asian Pacific Journal of Cancer Prevention : APJCP
Issue Date:
11-Mar-2009
URI:
http://hdl.handle.net/10146/54153
PubMed ID:
16839232
Additional Links:
http://www.apocp.org/journal_of_cancer_prevention_volume_7.php
Type:
Article
Language:
en
Description:
Biomarkers of individual susceptibility: field studies. Biomarker: GSTM1, GSTT1 and GSTP1 polymorphisms. Effect studied: DNA damageTissue/biological material/sample size: blood. Method of analysis: GSTM1 and GSTT1 genotypes were determined by PCR and GSTP1 by PCR-RFLPStudy design: cross-sectional studyStudy size: 255 healthy random volunteersImpact on outcome (including dose-response): The GSTM1 and GSTT1 null genotype frequencies were found to be 22.4% and 17.6% respectively. The GSTP1 allelic frequency was 0.78 for the Ile allele and 0.22 for the Val allele and the genotype frequency was 58.4% for Ile/Ile, 38.4% for Ile/Val, and 3.1% for Val/Val. KEYWORDS- - CLASSIFICATION: Adult;Aged;Alleles;analysis;biomarkers of individual susceptibility: field studies;blood;Ethnic Groups;Female;field studies;genetic;genetics;Genotype;Glutathione;Glutathione S-Transferase pi;Glutathione Transferase;Human;Humans;India;Male;Middle Aged;Polymorphism,Genetic;Reference Values;Research;
ISSN:
1513-7368
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorVettriselvi, V.-
dc.contributor.authorVijayalakshmi, K.-
dc.contributor.authorSolomon Fd, Paul-
dc.contributor.authorVenkatachalam, P.-
dc.date.accessioned2009-03-11T10:14:54Z-
dc.date.available2009-03-11T10:14:54Z-
dc.date.issued2009-03-11T10:14:54Z-
dc.identifier.citationAsian Pac. J. Cancer Prev., 7 (2):325-328en
dc.identifier.issn1513-7368-
dc.identifier.pmid16839232-
dc.identifier.urihttp://hdl.handle.net/10146/54153-
dc.descriptionBiomarkers of individual susceptibility: field studies. Biomarker: GSTM1, GSTT1 and GSTP1 polymorphisms. Effect studied: DNA damageTissue/biological material/sample size: blood. Method of analysis: GSTM1 and GSTT1 genotypes were determined by PCR and GSTP1 by PCR-RFLPStudy design: cross-sectional studyStudy size: 255 healthy random volunteersImpact on outcome (including dose-response): The GSTM1 and GSTT1 null genotype frequencies were found to be 22.4% and 17.6% respectively. The GSTP1 allelic frequency was 0.78 for the Ile allele and 0.22 for the Val allele and the genotype frequency was 58.4% for Ile/Ile, 38.4% for Ile/Val, and 3.1% for Val/Val. KEYWORDS- - CLASSIFICATION: Adult;Aged;Alleles;analysis;biomarkers of individual susceptibility: field studies;blood;Ethnic Groups;Female;field studies;genetic;genetics;Genotype;Glutathione;Glutathione S-Transferase pi;Glutathione Transferase;Human;Humans;India;Male;Middle Aged;Polymorphism,Genetic;Reference Values;Research;en
dc.description.abstractThe glutathione S transferase (GST) family of enzymes play a vital role in the phase II biotransformation of environmental carcinogens, pollutants, drugs and other xenobiotics. GSTs are polymorphic and the polymorphisms in GST genes have been associated with cancer susceptibility and prognosis. Moreover, distinct ethnic differences have been observed in the type and frequency of GST gene polymorphisms. Hence, the present study was aimed to determine the frequencies of GSTM1, GSTT1 and GSTP1 polymorphisms in 255 healthy random volunteers from South India. The GSTM1 and GSTT1 genotypes were determined by PCR and GSTP1 by PCR-RFLP using peripheral blood DNA.The GSTM1 and GSTT1 null genotype frequencies were found to be 22.4% and 17.6% respectively. The GSTP1 allelic frequency was 0.78 for the Ile allele and 0.22 for the Val allele and the genotype frequency was 58.4% for Ile/Ile, 38.4% for Ile/Val, and 3.1% for Val/Val. Comparison of the frequencies of GST polymorphisms observed in the present study with other Indian and world populations revealed a distinctive nature of the South Indian population with respect to polymorphims at the GST gene loci. A better understanding of carcinogen metabolizing gene distribution should contribute to risk assessment of humans exposed to environmental carcinogens.en
dc.language.isoenen
dc.relation.urlhttp://www.apocp.org/journal_of_cancer_prevention_volume_7.phpen
dc.subjectGSTM1en
dc.subjectGSTT1en
dc.subjectGSTP1en
dc.subjectpolymorphismsen
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshEthnic Groups-
dc.subject.meshFemale-
dc.subject.meshGenotype-
dc.subject.meshGlutathione S-Transferase pi-
dc.subject.meshGlutathione Transferase-
dc.subject.meshHumans-
dc.subject.meshIndia-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshPolymorphism, Genetic-
dc.subject.meshReference Values-
dc.titleGenetic variation of GSTM1, GSTT1 and GSTP1 genes in a South Indian population.en
dc.typeArticleen
dc.identifier.journalAsian Pacific Journal of Cancer Prevention : APJCPen

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