Carbohydrate digestibility predicts colon carcinogenesis in azoxymethane-treated rats.

2.50
Hdl Handle:
http://hdl.handle.net/10146/52553
Title:
Carbohydrate digestibility predicts colon carcinogenesis in azoxymethane-treated rats.
Authors:
Jacobsen, Helene; Poulsen, Morten; Dragsted, Lars Ove; Ravn-Haren, Gitte; Meyer, Otto; Lindecrona, Rikke Hvid
Abstract:
The purpose of this study was to compare the effect of carbohydrate structure and digestibility on azoxymethane (AOM)-induced colon carcinogenesis. Five groups of male Fischer 344 rats each comprising 30 animals were injected with AOM and fed a high-fat diet with 15% of various carbohydrates. The carbohydrate sources used were sucrose, cornstarch (a linear starch, reference group), potato starch (a branched starch), a short-chained oligofructose (Raftilose), and a long-chained inulin-type fructan (Raftiline). An interim sacrifice was performed after 9 wk to investigate markers of carbohydrate digestibility, including caecal fermentation (caecum weight and pH) and glucose and lipid metabolism [glucose, fructoseamine, HbA1c, triglycerides, and insulin-like growth factor (IGF) 1]. In addition potential early predictors of carcinogenicity [cell proliferation and aberrant crypt foci (ACF)] at 9 wk and their correlation to colon cancer risk after 32 wk were investigated. Tumor incidence was significantly reduced in animals fed oligofructose, and the number of tumors per animal was significantly reduced in animals fed inulin and oligofructose at 32 wk after AOM induction compared to the reference group fed sucrose. Increased caecum weight and decreased caecal pH were seen in groups fed oligofructose, inulin, and potato starch. Plasma triglyceride was decreased in rats fed oligofructose and inulin. Cell proliferation was increased in the proximal colon of rats fed sucrose, oligofructose, and inulin, and the number of cells per crypt decreased in rats fed oligofructose and inulin. The total number of ACF's was unaffected by treatment, and the size and multiplicity of ACF was unrelated to tumor development. It was concluded that less digestible carbohydrates with an early effect on caecum fermentation and plasma triglyceride decreased subsequent tumor incidence and multiplicity. This was unrelated to ACF, cell proliferation, and other markers of glucose and lipid metabolism.
Citation:
Nutr. Cancer 2006, 55 (2):163-170
Journal:
Nutrition and Cancer
Issue Date:
2006
URI:
http://hdl.handle.net/10146/52553
DOI:
10.1207/s15327914nc5502_7
PubMed ID:
17044771
Additional Links:
http://www.informaworld.com/smpp/content~content=a785830115~db=all~order=page
Type:
Article
Language:
en
Description:
Dietary modulation of cancer & cancer biomarkersDietary item or component studied: carbohydrates. Outcome studied: colon cancer risk; colon cell proliferation; aberrant crypt foci (ACF) Study type: male Fischer 344 rats Tissue/biological material/sample size: colon. Mode of exposure: dietary. Impact on outcome (including dose-response): Tumor incidence was significantly reduced in animals fed oligofructose, and the number of tumors per animal was significantly reduced in animals fed inulin and oligofructose at 32 wk after AOM induction compared to the reference group fed sucrose. Increased caecum weight and decreased caecal pH were seen in groups fed oligofructose, inulin, and potato starch. Plasma triglyceride was decreased in rats fed oligofructose and inulin. Cell proliferation was increased in the proximal colon of rats fed sucrose, oligofructose, and inulin, and the number of cells per crypt decreased in rats fed oligofructose and inulin. The total number of ACF's was unaffected by treatment, and the size and multiplicity of ACF was unrelated to tumor development. Keywords - classification: administration & dosage;Animals;Azoxymethane;blood;cancer epidemiology;Carcinogens;Cecum;Colonic Neoplasms;Denmark;Dietary Carbohydrates;Dietary Fats;Dietary Fiber;dietary modulation of cancer & cancer biomarkers;Digestion;Disease Models,Animal;epidemiology;Food;Fructose;humans;Hydrogen-Ion Concentration;Inulin;lifestyle modulation of cancer & cancer biomarkers;Lipid Metabolism;Male;metabolism;microbiology;Oligosaccharides;Organ Size;Plasma;Precancerous Conditions;prevention & control;Random Allocation;Rats;Rats,Inbred F344;Research;Solubility;toxicity;Triglycerides;
ISSN:
0163-5581
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorJacobsen, Helene-
dc.contributor.authorPoulsen, Morten-
dc.contributor.authorDragsted, Lars Ove-
dc.contributor.authorRavn-Haren, Gitte-
dc.contributor.authorMeyer, Otto-
dc.contributor.authorLindecrona, Rikke Hvid-
dc.date.accessioned2009-03-06T10:07:13Z-
dc.date.available2009-03-06T10:07:13Z-
dc.date.issued2006-
dc.identifier.citationNutr. Cancer 2006, 55 (2):163-170en
dc.identifier.issn0163-5581-
dc.identifier.pmid17044771-
dc.identifier.doi10.1207/s15327914nc5502_7-
dc.identifier.urihttp://hdl.handle.net/10146/52553-
dc.descriptionDietary modulation of cancer & cancer biomarkersDietary item or component studied: carbohydrates. Outcome studied: colon cancer risk; colon cell proliferation; aberrant crypt foci (ACF) Study type: male Fischer 344 rats Tissue/biological material/sample size: colon. Mode of exposure: dietary. Impact on outcome (including dose-response): Tumor incidence was significantly reduced in animals fed oligofructose, and the number of tumors per animal was significantly reduced in animals fed inulin and oligofructose at 32 wk after AOM induction compared to the reference group fed sucrose. Increased caecum weight and decreased caecal pH were seen in groups fed oligofructose, inulin, and potato starch. Plasma triglyceride was decreased in rats fed oligofructose and inulin. Cell proliferation was increased in the proximal colon of rats fed sucrose, oligofructose, and inulin, and the number of cells per crypt decreased in rats fed oligofructose and inulin. The total number of ACF's was unaffected by treatment, and the size and multiplicity of ACF was unrelated to tumor development. Keywords - classification: administration & dosage;Animals;Azoxymethane;blood;cancer epidemiology;Carcinogens;Cecum;Colonic Neoplasms;Denmark;Dietary Carbohydrates;Dietary Fats;Dietary Fiber;dietary modulation of cancer & cancer biomarkers;Digestion;Disease Models,Animal;epidemiology;Food;Fructose;humans;Hydrogen-Ion Concentration;Inulin;lifestyle modulation of cancer & cancer biomarkers;Lipid Metabolism;Male;metabolism;microbiology;Oligosaccharides;Organ Size;Plasma;Precancerous Conditions;prevention & control;Random Allocation;Rats;Rats,Inbred F344;Research;Solubility;toxicity;Triglycerides;en
dc.description.abstractThe purpose of this study was to compare the effect of carbohydrate structure and digestibility on azoxymethane (AOM)-induced colon carcinogenesis. Five groups of male Fischer 344 rats each comprising 30 animals were injected with AOM and fed a high-fat diet with 15% of various carbohydrates. The carbohydrate sources used were sucrose, cornstarch (a linear starch, reference group), potato starch (a branched starch), a short-chained oligofructose (Raftilose), and a long-chained inulin-type fructan (Raftiline). An interim sacrifice was performed after 9 wk to investigate markers of carbohydrate digestibility, including caecal fermentation (caecum weight and pH) and glucose and lipid metabolism [glucose, fructoseamine, HbA1c, triglycerides, and insulin-like growth factor (IGF) 1]. In addition potential early predictors of carcinogenicity [cell proliferation and aberrant crypt foci (ACF)] at 9 wk and their correlation to colon cancer risk after 32 wk were investigated. Tumor incidence was significantly reduced in animals fed oligofructose, and the number of tumors per animal was significantly reduced in animals fed inulin and oligofructose at 32 wk after AOM induction compared to the reference group fed sucrose. Increased caecum weight and decreased caecal pH were seen in groups fed oligofructose, inulin, and potato starch. Plasma triglyceride was decreased in rats fed oligofructose and inulin. Cell proliferation was increased in the proximal colon of rats fed sucrose, oligofructose, and inulin, and the number of cells per crypt decreased in rats fed oligofructose and inulin. The total number of ACF's was unaffected by treatment, and the size and multiplicity of ACF was unrelated to tumor development. It was concluded that less digestible carbohydrates with an early effect on caecum fermentation and plasma triglyceride decreased subsequent tumor incidence and multiplicity. This was unrelated to ACF, cell proliferation, and other markers of glucose and lipid metabolism.en
dc.language.isoenen
dc.relation.urlhttp://www.informaworld.com/smpp/content~content=a785830115~db=all~order=pageen
dc.subject.meshAnimals-
dc.subject.meshAzoxymethane-
dc.subject.meshCarcinogens-
dc.subject.meshCecum-
dc.subject.meshColonic Neoplasms-
dc.subject.meshDietary Carbohydrates-
dc.subject.meshDietary Fats-
dc.subject.meshDietary Fiber-
dc.subject.meshDigestion-
dc.subject.meshDisease Models, Animal-
dc.subject.meshFructose-
dc.subject.meshHydrogen-Ion Concentration-
dc.subject.meshInulin-
dc.subject.meshLipid Metabolism-
dc.subject.meshMale-
dc.subject.meshOligosaccharides-
dc.subject.meshOrgan Size-
dc.subject.meshPrecancerous Conditions-
dc.subject.meshRandom Allocation-
dc.subject.meshRats-
dc.subject.meshRats, Inbred F344-
dc.subject.meshSolubility-
dc.subject.meshTriglycerides-
dc.titleCarbohydrate digestibility predicts colon carcinogenesis in azoxymethane-treated rats.en
dc.typeArticleen
dc.identifier.journalNutrition and Canceren
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