Assessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions.

4.00
Hdl Handle:
http://hdl.handle.net/10146/51913
Title:
Assessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions.
Authors:
Bolognesi, Claudia; Perrone, Emanuela; Roggieri, Paola; Pampanin, Daniela M.; Sciutto, Andrea
Abstract:
The aim of the present study was to standardize and to assess the predictive value of the cytogenetic analysis by MN test in fish erythrocytes as a biomarker for marine environmental contamination. MN frequency baseline in erythrocytes was evaluated in a number of fish species from a reference area (S. Teresa, La Spezia Gulf) and genotoxic potential of a number of common chemical contaminants and mixtures was determined in fish experimentally exposed in aquarium under controlled conditions. Fish (Scophthalmus maximus) were exposed for 3 weeks to 50 ppb of single chemicals (dialkyl phthalate, bisphenol A, tetrabromodiphenyl ether), 30 ppb nonylphenol and mixtures (North Sea oil and North Sea oil with alkylated phenols). Chromosomal damage was determined as micronuclei (MN) frequency in fish erythrocytes. Nuclear anomalies such as blebbed, notched and lobed nuclei were also recorded. Significant increase in MN frequency was observed in erythrocytes of fish exposed to bisphenol A and tetrabromodiphenylether. Chemical mixture North Sea oil+alkylated phenols induced the highest MN frequency (2.95 micronucleated cells/1000 cells compared to 1 MNcell/1000 cells in control animals). The study results revealed that micronucleus test, as an index of cumulative exposure, appears to be a sensitive model to evaluate genotoxic compounds in fish under controlled conditions.
Citation:
Aquat. Toxicol. 2006, 78 Suppl 1:S93-S98
Journal:
Aquatic toxicology (Amsterdam, Netherlands)
Issue Date:
1-Jun-2006
URI:
http://hdl.handle.net/10146/51913
DOI:
10.1016/j.aquatox.2006.02.015
PubMed ID:
16600396
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T4G-4JMVHFT-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=00be1730fc7d7161b81686b82322aa2f
Type:
Article
Language:
en
Description:
Biomarkers of exposure & effect:: validationBiomarker: MNExposure/effect represented: genetic damageStudy type (in vitro, animals, humans): fishMode of exposure (if in vivo) (acute, chronic, root of exposure):chronicTissue/biological material/sample size:4*103 erythrocytes/animalMethod of analysis: MN testSensitivity (LOD): low baseline frequencyIntra-individual variation: large range of variabilityInter-individual variation:0-2 MN frequencyDose-response: 2.95 MN/1000 cells compared to 1MN/1000 cells in the control, p<0.005 Keywords classification: analysis;biomarkers of exposure & effect: validation;Italy;Research;validation;humans;genetic;
ISSN:
0166-445X
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorBolognesi, Claudia-
dc.contributor.authorPerrone, Emanuela-
dc.contributor.authorRoggieri, Paola-
dc.contributor.authorPampanin, Daniela M.-
dc.contributor.authorSciutto, Andrea-
dc.date.accessioned2009-03-04T07:59:55Z-
dc.date.available2009-03-04T07:59:55Z-
dc.date.issued2006-06-01-
dc.identifier.citationAquat. Toxicol. 2006, 78 Suppl 1:S93-S98en
dc.identifier.issn0166-445X-
dc.identifier.pmid16600396-
dc.identifier.doi10.1016/j.aquatox.2006.02.015-
dc.identifier.urihttp://hdl.handle.net/10146/51913-
dc.descriptionBiomarkers of exposure & effect:: validationBiomarker: MNExposure/effect represented: genetic damageStudy type (in vitro, animals, humans): fishMode of exposure (if in vivo) (acute, chronic, root of exposure):chronicTissue/biological material/sample size:4*103 erythrocytes/animalMethod of analysis: MN testSensitivity (LOD): low baseline frequencyIntra-individual variation: large range of variabilityInter-individual variation:0-2 MN frequencyDose-response: 2.95 MN/1000 cells compared to 1MN/1000 cells in the control, p<0.005 Keywords classification: analysis;biomarkers of exposure & effect: validation;Italy;Research;validation;humans;genetic;en
dc.description.abstractThe aim of the present study was to standardize and to assess the predictive value of the cytogenetic analysis by MN test in fish erythrocytes as a biomarker for marine environmental contamination. MN frequency baseline in erythrocytes was evaluated in a number of fish species from a reference area (S. Teresa, La Spezia Gulf) and genotoxic potential of a number of common chemical contaminants and mixtures was determined in fish experimentally exposed in aquarium under controlled conditions. Fish (Scophthalmus maximus) were exposed for 3 weeks to 50 ppb of single chemicals (dialkyl phthalate, bisphenol A, tetrabromodiphenyl ether), 30 ppb nonylphenol and mixtures (North Sea oil and North Sea oil with alkylated phenols). Chromosomal damage was determined as micronuclei (MN) frequency in fish erythrocytes. Nuclear anomalies such as blebbed, notched and lobed nuclei were also recorded. Significant increase in MN frequency was observed in erythrocytes of fish exposed to bisphenol A and tetrabromodiphenylether. Chemical mixture North Sea oil+alkylated phenols induced the highest MN frequency (2.95 micronucleated cells/1000 cells compared to 1 MNcell/1000 cells in control animals). The study results revealed that micronucleus test, as an index of cumulative exposure, appears to be a sensitive model to evaluate genotoxic compounds in fish under controlled conditions.en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T4G-4JMVHFT-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=00be1730fc7d7161b81686b82322aa2fen
dc.subjectScophthalmus maximusen
dc.subjectXenobioticsen
dc.subjectMN frequencyen
dc.subjectBiomarkeren
dc.subjectFish erythrocytesen
dc.subject.meshAnimals-
dc.subject.meshCell Nucleus-
dc.subject.meshErythrocytes-
dc.subject.meshFishes-
dc.subject.meshFlatfishes-
dc.subject.meshMicronuclei, Chromosome-Defective-
dc.subject.meshMicronucleus Tests-
dc.subject.meshOrganic Chemicals-
dc.subject.meshPetroleum-
dc.subject.meshPredictive Value of Tests-
dc.subject.meshStatistics as Topic-
dc.subject.meshWater Pollutants, Chemical-
dc.subject.meshXenobiotics-
dc.titleAssessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions.en
dc.typeArticleen
dc.identifier.journalAquatic toxicology (Amsterdam, Netherlands)en

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