The flavouring phytochemical 2-pentanone reduces prostaglandin production and COX-2 expression in colon cancer cells.

2.50
Hdl Handle:
http://hdl.handle.net/10146/37193
Title:
The flavouring phytochemical 2-pentanone reduces prostaglandin production and COX-2 expression in colon cancer cells.
Authors:
Pettersson, Jenny; Karlsson, Pernilla Christina; Goransson, Ulf; Rafter, Joseph James; Bohlin, Lars
Abstract:
Many phytochemicals found in the diet may prevent colon carcinogenesis by affecting biochemical processes in the colonic mucosa. Inflammation and subsequent elevation of the enzyme cyclooxygenase-2 (COX-2) are two such factors involved in the development of colon cancer, and inhibition of these processes could be important targets for chemoprevention. We have previously shown COX-2 inhibitory activity locally in the colon; e.g. in human fecal water from a group of vegetarians. In this study we focus on 2-pentanone, a frequently occurring compound in common foods such as banana and carrot. The aim was to study the inhibitory effects on prostaglandin production and COX-2 protein expression in tumour necrosis factor-alpha stimulated colon cancer cells (HT29) by radioimmunoassay and Western blotting. 2-Pentanone inhibited both prostaglandin production and COX-2 protein expression in human colon cancer cells. A concentration of 400 mumol/l 2-pentanone inhibited the prostaglandin production by 56.9+/-12.9% which is in the same range as the reference compound NS398 (59.8+/-7.6%). The two highest concentrations of 2-pentanone were further analyzed by Western blot, and 400 micromol/l and 200 micromol/l 2-pentanone resulted in a 53.3+/-9.6% and +/-27.1% reduction of the COX-2 protein levels respectively. Further studies on flavouring compounds, for example 2-pentanone, as colon cancer chemopreventives would be very valuable, and such results may contribute to future dietary recommendations.
Citation:
Biol. Pharm. Bull.. 2008, 31 (3):534-537
Journal:
Biological & Pharmaceutical Bulletin
Issue Date:
Mar-2008
URI:
http://hdl.handle.net/10146/37193
PubMed ID:
18310926
Additional Links:
http://www.jstage.jst.go.jp/article/bpb/31/3/31_534/_article
Type:
Article
Language:
en
ISSN:
0918-6158
Sponsors:
Supported by grants from Agricultural Sciences and Spatial Planning (FORMAS), Swedish Cancer Society, and Stiftelsen Ruth och Richard Julins Fond. This work was partly supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943). Special thanks are due to Uddeholms Aktiebolags stipendiefond for the grant to Jenny Pettersson. The authors also want to thank Dr. Ulrika Huss for helpful discussion.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorPettersson, Jenny-
dc.contributor.authorKarlsson, Pernilla Christina-
dc.contributor.authorGoransson, Ulf-
dc.contributor.authorRafter, Joseph James-
dc.contributor.authorBohlin, Lars-
dc.date.accessioned2008-09-04T08:52:52Z-
dc.date.available2008-09-04T08:52:52Z-
dc.date.issued2008-03-
dc.identifier.citationBiol. Pharm. Bull.. 2008, 31 (3):534-537en
dc.identifier.issn0918-6158-
dc.identifier.pmid18310926-
dc.identifier.urihttp://hdl.handle.net/10146/37193-
dc.description.abstractMany phytochemicals found in the diet may prevent colon carcinogenesis by affecting biochemical processes in the colonic mucosa. Inflammation and subsequent elevation of the enzyme cyclooxygenase-2 (COX-2) are two such factors involved in the development of colon cancer, and inhibition of these processes could be important targets for chemoprevention. We have previously shown COX-2 inhibitory activity locally in the colon; e.g. in human fecal water from a group of vegetarians. In this study we focus on 2-pentanone, a frequently occurring compound in common foods such as banana and carrot. The aim was to study the inhibitory effects on prostaglandin production and COX-2 protein expression in tumour necrosis factor-alpha stimulated colon cancer cells (HT29) by radioimmunoassay and Western blotting. 2-Pentanone inhibited both prostaglandin production and COX-2 protein expression in human colon cancer cells. A concentration of 400 mumol/l 2-pentanone inhibited the prostaglandin production by 56.9+/-12.9% which is in the same range as the reference compound NS398 (59.8+/-7.6%). The two highest concentrations of 2-pentanone were further analyzed by Western blot, and 400 micromol/l and 200 micromol/l 2-pentanone resulted in a 53.3+/-9.6% and +/-27.1% reduction of the COX-2 protein levels respectively. Further studies on flavouring compounds, for example 2-pentanone, as colon cancer chemopreventives would be very valuable, and such results may contribute to future dietary recommendations.en
dc.description.sponsorshipSupported by grants from Agricultural Sciences and Spatial Planning (FORMAS), Swedish Cancer Society, and Stiftelsen Ruth och Richard Julins Fond. This work was partly supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943). Special thanks are due to Uddeholms Aktiebolags stipendiefond for the grant to Jenny Pettersson. The authors also want to thank Dr. Ulrika Huss for helpful discussion.en
dc.language.isoenen
dc.relation.urlhttp://www.jstage.jst.go.jp/article/bpb/31/3/31_534/_articleen
dc.subjectcyclooxygenase-2 inhibitoren
dc.subjectprostaglandin E2en
dc.subject2-pentanoneen
dc.subjectinflammationen
dc.subjectcolon cancer cellen
dc.subject.meshBlotting, Western-
dc.subject.meshCell Line, Tumor-
dc.subject.meshColonic Neoplasms-
dc.subject.meshCyclooxygenase 2-
dc.subject.meshCyclooxygenase 2 Inhibitors-
dc.subject.meshDiet, Vegetarian-
dc.subject.meshDinoprostone-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshFlavoring Agents-
dc.subject.meshHumans-
dc.subject.meshPentanones-
dc.titleThe flavouring phytochemical 2-pentanone reduces prostaglandin production and COX-2 expression in colon cancer cells.en
dc.typeArticleen
dc.identifier.journalBiological & Pharmaceutical Bulletinen

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