NMR metabolomic analysis of fecal water from subjects on a vegetarian diet.

2.50
Hdl Handle:
http://hdl.handle.net/10146/37192
Title:
NMR metabolomic analysis of fecal water from subjects on a vegetarian diet.
Authors:
Pettersson, Jenny; Karlsson, Pernilla Christina; Choi, Young Hae; Verpoorte, Robert; Rafter, Joseph James; Bohlin, Lars
Abstract:
A vegetarian diet rich in phytochemicals may prevent colon carcinogenesis by affecting biochemical processes in the colonic mucosa. Compounds passing the digestive system reaching the colon could potentially be detected in fecal water. We previously reported that intact fecal water samples from human volunteers significantly decreased prostaglandin production and COX-2 protein expression in colonic cells. The aim with the present study was to further study the composition of the fecal waters, using NMR spectroscopy and multivariate data analysis, and to trace the COX-2 inhibiting activity. Intact fecal water samples and fractions thereof were analyzed for their ability to inhibit prostaglandin E2 production in the human colon cell line HT-29. The majority of the tested aqueous phases derived from intact fecal water showed ability to inhibit prostaglandin production in cells (13.8+/-1.34% inhibition, p=0.01). NMR analysis indicated the presence of significant quantities of amino acids and fatty acids. Major metabolites included; acetic acid, butanoic acid, propanoic acid, glutamic acid and alanine. Smaller amounts of glycine and fumaric acid, which are known to have anti-inflammatory and anti-tumorigenic properties, were also detected. This study describes for the first time NMR metabolomic analysis of fecal water from subjects on a vegetarian diet.
Citation:
Biol. Pharm. Bull. 2008, 31 (6):1192-1198
Journal:
Biological & Pharmaceutical Bulletin
Issue Date:
Jun-2008
URI:
http://hdl.handle.net/10146/37192
PubMed ID:
18520053
Additional Links:
http://www.jstage.jst.go.jp/article/bpb/31/6/31_1192/_article
Type:
Article
Language:
en
ISSN:
0918-6158
Sponsors:
Our research is also partly supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorPettersson, Jenny-
dc.contributor.authorKarlsson, Pernilla Christina-
dc.contributor.authorChoi, Young Hae-
dc.contributor.authorVerpoorte, Robert-
dc.contributor.authorRafter, Joseph James-
dc.contributor.authorBohlin, Lars-
dc.date.accessioned2008-09-04T08:48:01Z-
dc.date.available2008-09-04T08:48:01Z-
dc.date.issued2008-06-
dc.identifier.citationBiol. Pharm. Bull. 2008, 31 (6):1192-1198en
dc.identifier.issn0918-6158-
dc.identifier.pmid18520053-
dc.identifier.urihttp://hdl.handle.net/10146/37192-
dc.description.abstractA vegetarian diet rich in phytochemicals may prevent colon carcinogenesis by affecting biochemical processes in the colonic mucosa. Compounds passing the digestive system reaching the colon could potentially be detected in fecal water. We previously reported that intact fecal water samples from human volunteers significantly decreased prostaglandin production and COX-2 protein expression in colonic cells. The aim with the present study was to further study the composition of the fecal waters, using NMR spectroscopy and multivariate data analysis, and to trace the COX-2 inhibiting activity. Intact fecal water samples and fractions thereof were analyzed for their ability to inhibit prostaglandin E2 production in the human colon cell line HT-29. The majority of the tested aqueous phases derived from intact fecal water showed ability to inhibit prostaglandin production in cells (13.8+/-1.34% inhibition, p=0.01). NMR analysis indicated the presence of significant quantities of amino acids and fatty acids. Major metabolites included; acetic acid, butanoic acid, propanoic acid, glutamic acid and alanine. Smaller amounts of glycine and fumaric acid, which are known to have anti-inflammatory and anti-tumorigenic properties, were also detected. This study describes for the first time NMR metabolomic analysis of fecal water from subjects on a vegetarian diet.en
dc.description.sponsorshipOur research is also partly supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).en
dc.language.isoenen
dc.relation.urlhttp://www.jstage.jst.go.jp/article/bpb/31/6/31_1192/_articleen
dc.subjectmetabolomicen
dc.subjectvegetarian fecal wateren
dc.subjectcyclooxygenase-2 inhibitoren
dc.subjectnuclear magnetic resonanceen
dc.subjectNMRen
dc.subjectcolon canceren
dc.subject.meshAnti-Inflammatory Agents, Non-Steroidal-
dc.subject.meshCyclooxygenase 2 Inhibitors-
dc.subject.meshDiet, Vegetarian-
dc.subject.meshDinoprostone-
dc.subject.meshFatty Acids-
dc.subject.meshFeces-
dc.subject.meshHT29 Cells-
dc.subject.meshHumans-
dc.subject.meshMagnetic Resonance Spectroscopy-
dc.subject.meshMetabolism-
dc.subject.meshPassive Cutaneous Anaphylaxis-
dc.subject.meshTissue Extracts-
dc.subject.meshWater-
dc.titleNMR metabolomic analysis of fecal water from subjects on a vegetarian diet.en
dc.typeArticleen
dc.identifier.journalBiological & Pharmaceutical Bulletinen

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