Acute hypoxia and reoxygenation-induced DNA oxidation in human mononuclear blood cells.

2.50
Hdl Handle:
http://hdl.handle.net/10146/37173
Title:
Acute hypoxia and reoxygenation-induced DNA oxidation in human mononuclear blood cells.
Authors:
Risom, Lotte; Lundby, Carsten; Thomsen, Jonas Juhl; Mikkelsen, Lone; Loft, Steffen; Friis, Gitte; Moller, Peter
Abstract:
Research indicates that exposure to hypoxia is associated with oxidative stress. In this investigation, healthy subjects were exposed to hypoxia by inhalation of 10% oxygen for 2h (corresponding to 5500m above sea level). The levels of strand breaks and oxidatively damaged purine bases, measured by the comet assay, and the expression of genes involved in DNA repair of oxidatively damaged DNA were investigated in mononuclear blood cells (MNBC) at baseline, after 2h of hypoxia, 2h of reoxygenation, and 1 day and 8 days after the exposure. The level of strand breaks and oxidized purine bases in MNBC increased following both the 2h of hypoxia and the 2h reoxygenation period, whereas this effect was not observed in unexposed subjects. The expressions of oxoguanine DNA glycosylase 1 (OGG1), nucleoside diphosphate linked moiety X-type motif 1 (NUDT1), nei endonuclease VIII-like 1 (NEIL1), and mutY homolog (MUTYH) were unaltered throughout the experiment in both groups of subjects, indicating that DNA repair genes are not up-regulated by the hypoxia and reoxygenation treatment. Taken together, this report shows that inhalation of 10% oxygen for 2h is associated with increased number of oxidized DNA lesions in MNBC, but acute hypoxia may not inflict upon the regulation of genes involved in repair of oxidized DNA.
Citation:
Mutat. Res. 2007, 625 (1-2):125-133
Journal:
Mutation Research
Issue Date:
1-Dec-2007
URI:
http://hdl.handle.net/10146/37173
DOI:
10.1016/j.mrfmmm.2007.06.001
PubMed ID:
17644143
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2C-4NYSXWR-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=1843694&md5=bac0fdcafb9dbd3f60202229312e6a33
Type:
Article
Language:
en
ISSN:
0027-5107
Sponsors:
This study was supported by the Danish Research Agency. The authors (L.R., L.M., S.L., G.F., and P.M.) of this paper are partners of ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorRisom, Lotte-
dc.contributor.authorLundby, Carsten-
dc.contributor.authorThomsen, Jonas Juhl-
dc.contributor.authorMikkelsen, Lone-
dc.contributor.authorLoft, Steffen-
dc.contributor.authorFriis, Gitte-
dc.contributor.authorMoller, Peter-
dc.date.accessioned2008-09-04T09:01:02Z-
dc.date.available2008-09-04T09:01:02Z-
dc.date.issued2007-12-01-
dc.identifier.citationMutat. Res. 2007, 625 (1-2):125-133en
dc.identifier.issn0027-5107-
dc.identifier.pmid17644143-
dc.identifier.doi10.1016/j.mrfmmm.2007.06.001-
dc.identifier.urihttp://hdl.handle.net/10146/37173-
dc.description.abstractResearch indicates that exposure to hypoxia is associated with oxidative stress. In this investigation, healthy subjects were exposed to hypoxia by inhalation of 10% oxygen for 2h (corresponding to 5500m above sea level). The levels of strand breaks and oxidatively damaged purine bases, measured by the comet assay, and the expression of genes involved in DNA repair of oxidatively damaged DNA were investigated in mononuclear blood cells (MNBC) at baseline, after 2h of hypoxia, 2h of reoxygenation, and 1 day and 8 days after the exposure. The level of strand breaks and oxidized purine bases in MNBC increased following both the 2h of hypoxia and the 2h reoxygenation period, whereas this effect was not observed in unexposed subjects. The expressions of oxoguanine DNA glycosylase 1 (OGG1), nucleoside diphosphate linked moiety X-type motif 1 (NUDT1), nei endonuclease VIII-like 1 (NEIL1), and mutY homolog (MUTYH) were unaltered throughout the experiment in both groups of subjects, indicating that DNA repair genes are not up-regulated by the hypoxia and reoxygenation treatment. Taken together, this report shows that inhalation of 10% oxygen for 2h is associated with increased number of oxidized DNA lesions in MNBC, but acute hypoxia may not inflict upon the regulation of genes involved in repair of oxidized DNA.en
dc.description.sponsorshipThis study was supported by the Danish Research Agency. The authors (L.R., L.M., S.L., G.F., and P.M.) of this paper are partners of ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2C-4NYSXWR-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=1843694&md5=bac0fdcafb9dbd3f60202229312e6a33en
dc.subjectComet assayen
dc.subjectDNA repairen
dc.subjectGene expressionen
dc.subjectOxidative DNA damageen
dc.subject.meshAdult-
dc.subject.meshAnoxia-
dc.subject.meshBase Sequence-
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Glycosylases-
dc.subject.meshDNA Primers-
dc.subject.meshDNA Repair-
dc.subject.meshDNA Repair Enzymes-
dc.subject.meshFemale-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshLeukocytes, Mononuclear-
dc.subject.meshMale-
dc.subject.meshOxidative Stress-
dc.subject.meshPhosphoric Monoester Hydrolases-
dc.subject.meshRNA, Messenger-
dc.titleAcute hypoxia and reoxygenation-induced DNA oxidation in human mononuclear blood cells.en
dc.typeArticleen
dc.identifier.journalMutation Researchen

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