Separation of selenium, zinc, and copper compounds in bovine whey using size exclusion chromatography linked to inductively coupled plasma mass spectrometry.

2.50
Hdl Handle:
http://hdl.handle.net/10146/36653
Title:
Separation of selenium, zinc, and copper compounds in bovine whey using size exclusion chromatography linked to inductively coupled plasma mass spectrometry.
Authors:
Hoac, Tien; Lundh, Thomas; Purup, Stig; Onning, Gunilla; Sejrsen, Kristen; Akesson, Bjorn
Abstract:
To study the role of trace elements for the quality and nutritional value of bovine milk, the distribution of selenium, zinc, and copper in whey was investigated using a method linking size exclusion chromatography to inductively coupled plasma mass spectrometry (SEC-ICP-MS). Three major peaks were detected for selenium, two peaks for zinc, and five peaks for copper. More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. All zinc was associated with low molecular weight compounds (<5 kDa) and one of these compounds was probably citrate. More than 60% of the copper eluted in protein fractions and two of the five major peaks probably contained metallothionein and citrate. This method was used to compare milk and whey produced by organic and conventional feeding procedures. The selenium content in whey and desalted milk produced using organic regimens was significantly lower than that in conventional samples. Moreover, the proportion of selenium in protein fractions of organic whey was significantly smaller than that in conventional whey, but the distributions of zinc and copper did not differ. This study showed that with the SEC-ICP-MS technique the distribution profiles of several trace elements in whey could be studied in the same run and that the selenium profile differed in whey produced by organic and conventional procedures.
Citation:
J. Agric. Food Chem. 2007, 55 (10):4237-4243
Journal:
Journal of Agricultural and Food Chemistry
Issue Date:
16-May-2007
URI:
http://hdl.handle.net/10146/36653
DOI:
10.1021/jf070169x
PubMed ID:
17447791
Additional Links:
http://pubs.acs.org/cgi-bin/abstract.cgi/jafcau/2007/55/i10/abs/jf070169x.html
Type:
Article
Language:
en
ISSN:
0021-8561
Sponsors:
Biomedical Nutrition is a member of the EU NoE, The European Nutrigenomics Organisation (NuGO, no. 505944), and the NoE Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorHoac, Tien-
dc.contributor.authorLundh, Thomas-
dc.contributor.authorPurup, Stig-
dc.contributor.authorOnning, Gunilla-
dc.contributor.authorSejrsen, Kristen-
dc.contributor.authorAkesson, Bjorn-
dc.date.accessioned2008-08-27T12:46:15Z-
dc.date.available2008-08-27T12:46:15Z-
dc.date.issued2007-05-16-
dc.identifier.citationJ. Agric. Food Chem. 2007, 55 (10):4237-4243en
dc.identifier.issn0021-8561-
dc.identifier.pmid17447791-
dc.identifier.doi10.1021/jf070169x-
dc.identifier.urihttp://hdl.handle.net/10146/36653-
dc.description.abstractTo study the role of trace elements for the quality and nutritional value of bovine milk, the distribution of selenium, zinc, and copper in whey was investigated using a method linking size exclusion chromatography to inductively coupled plasma mass spectrometry (SEC-ICP-MS). Three major peaks were detected for selenium, two peaks for zinc, and five peaks for copper. More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. All zinc was associated with low molecular weight compounds (<5 kDa) and one of these compounds was probably citrate. More than 60% of the copper eluted in protein fractions and two of the five major peaks probably contained metallothionein and citrate. This method was used to compare milk and whey produced by organic and conventional feeding procedures. The selenium content in whey and desalted milk produced using organic regimens was significantly lower than that in conventional samples. Moreover, the proportion of selenium in protein fractions of organic whey was significantly smaller than that in conventional whey, but the distributions of zinc and copper did not differ. This study showed that with the SEC-ICP-MS technique the distribution profiles of several trace elements in whey could be studied in the same run and that the selenium profile differed in whey produced by organic and conventional procedures.en
dc.description.sponsorshipBiomedical Nutrition is a member of the EU NoE, The European Nutrigenomics Organisation (NuGO, no. 505944), and the NoE Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.en
dc.language.isoenen
dc.relation.urlhttp://pubs.acs.org/cgi-bin/abstract.cgi/jafcau/2007/55/i10/abs/jf070169x.htmlen
dc.subjectTrace element distributionen
dc.subjectICP-MSen
dc.subjectmilken
dc.subjectwheyen
dc.subjectseleniumen
dc.subjectcopperen
dc.subjectzincen
dc.subject.meshAnimals-
dc.subject.meshCattle-
dc.subject.meshChromatography, Gel-
dc.subject.meshCopper-
dc.subject.meshFemale-
dc.subject.meshMass Spectrometry-
dc.subject.meshMilk Proteins-
dc.subject.meshSelenium Compounds-
dc.subject.meshZinc Compounds-
dc.titleSeparation of selenium, zinc, and copper compounds in bovine whey using size exclusion chromatography linked to inductively coupled plasma mass spectrometry.en
dc.typeArticleen
dc.identifier.journalJournal of Agricultural and Food Chemistryen
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