Expression of DNA repair and metabolic genes in response to a flavonoid-rich diet.

2.50
Hdl Handle:
http://hdl.handle.net/10146/36573
Title:
Expression of DNA repair and metabolic genes in response to a flavonoid-rich diet.
Authors:
Guarrera, Simonetta; Sacerdote, Carlotta; Fiorini, Laura; Marsala, Rosa; Polidoro, Silvia; Gamberini, Sara; Saletta, Federica; Malaveille, Christian; Talaska, Glenn; Vineis, Paolo; Matullo, Giuseppe
Abstract:
A diet rich in fruit and vegetables can be effective in the reduction of oxidative stress, through the antioxidant effects of phytochemicals and other mechanisms. Protection against the carcinogenic effects of chemicals may also be exerted by an enhancement of detoxification and DNA damage repair mechanisms. To investigate a putative effect of flavonoids, a class of polyphenols, on the regulation of the gene expression of DNA repair and metabolic genes, a 1-month flavonoid-rich diet was administered to thirty healthy male smokers, nine of whom underwent gene expression analysis. We postulated that tobacco smoke is a powerful source of reactive oxygen species. The expression level of twelve genes (APEX, ERCC1, ERCC2, ERCC4, MGMT, OGG1, XPA, XPC, XRCC1, XRCC3, AHR, CYP1A1) was investigated. We found a significant increase (P < 0.001) in flavonoid intake. Urinary phenolic content and anti-mutagenicity did not significantly change after diet, nor was a correlation found between flavonoid intake and urinary phenolic levels or anti-mutagenicity. Phenolic levels showed a significant positive correlation with urinary anti-mutagenicity. AHR levels were significantly reduced after the diet (P = 0.038), whereas the other genes showed a generalized up regulation, significant for XRCC3 gene (P = 0.038). Also in the context of a generalized up regulation of DNA repair genes, we found a non-significant negative correlation between flavonoid intake and the expression of all the DNA repair genes. Larger studies are needed to clarify the possible effects of flavonoids in vivo; our preliminary results could help to better plan new studies on gene expression and diet.
Citation:
Br. J. Nutr. 2007, 98 (3):525-533
Journal:
The British Journal of Nutrition
Issue Date:
Sep-2007
URI:
http://hdl.handle.net/10146/36573
DOI:
10.1017/S0007114507725151
PubMed ID:
17445347
Additional Links:
http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1304576
Type:
Article
Language:
en
ISSN:
0007-1145
Sponsors:
Supported by the World Cancer Research Fund, Compagnia di San Paolo (Torino), Italian Association of Cancer Research (AIRC) and a grant of the European Commission for the ECNIS project (FOOD-CT-2005-513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGuarrera, Simonetta-
dc.contributor.authorSacerdote, Carlotta-
dc.contributor.authorFiorini, Laura-
dc.contributor.authorMarsala, Rosa-
dc.contributor.authorPolidoro, Silvia-
dc.contributor.authorGamberini, Sara-
dc.contributor.authorSaletta, Federica-
dc.contributor.authorMalaveille, Christian-
dc.contributor.authorTalaska, Glenn-
dc.contributor.authorVineis, Paolo-
dc.contributor.authorMatullo, Giuseppe-
dc.date.accessioned2008-08-27T11:19:50Z-
dc.date.available2008-08-27T11:19:50Z-
dc.date.issued2007-09-
dc.identifier.citationBr. J. Nutr. 2007, 98 (3):525-533en
dc.identifier.issn0007-1145-
dc.identifier.pmid17445347-
dc.identifier.doi10.1017/S0007114507725151-
dc.identifier.urihttp://hdl.handle.net/10146/36573-
dc.description.abstractA diet rich in fruit and vegetables can be effective in the reduction of oxidative stress, through the antioxidant effects of phytochemicals and other mechanisms. Protection against the carcinogenic effects of chemicals may also be exerted by an enhancement of detoxification and DNA damage repair mechanisms. To investigate a putative effect of flavonoids, a class of polyphenols, on the regulation of the gene expression of DNA repair and metabolic genes, a 1-month flavonoid-rich diet was administered to thirty healthy male smokers, nine of whom underwent gene expression analysis. We postulated that tobacco smoke is a powerful source of reactive oxygen species. The expression level of twelve genes (APEX, ERCC1, ERCC2, ERCC4, MGMT, OGG1, XPA, XPC, XRCC1, XRCC3, AHR, CYP1A1) was investigated. We found a significant increase (P < 0.001) in flavonoid intake. Urinary phenolic content and anti-mutagenicity did not significantly change after diet, nor was a correlation found between flavonoid intake and urinary phenolic levels or anti-mutagenicity. Phenolic levels showed a significant positive correlation with urinary anti-mutagenicity. AHR levels were significantly reduced after the diet (P = 0.038), whereas the other genes showed a generalized up regulation, significant for XRCC3 gene (P = 0.038). Also in the context of a generalized up regulation of DNA repair genes, we found a non-significant negative correlation between flavonoid intake and the expression of all the DNA repair genes. Larger studies are needed to clarify the possible effects of flavonoids in vivo; our preliminary results could help to better plan new studies on gene expression and diet.en
dc.description.sponsorshipSupported by the World Cancer Research Fund, Compagnia di San Paolo (Torino), Italian Association of Cancer Research (AIRC) and a grant of the European Commission for the ECNIS project (FOOD-CT-2005-513943).en
dc.language.isoenen
dc.relation.urlhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1304576en
dc.subjectGene expressionen
dc.subjectflavonoidsen
dc.subjectDietary intervention trialen
dc.subjectDNA repairen
dc.subject.meshAdult-
dc.subject.meshAntioxidants-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshDiet-
dc.subject.meshEating-
dc.subject.meshFlavonoids-
dc.subject.meshFood Analysis-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMutagenicity Tests-
dc.subject.meshPhenols-
dc.subject.meshSmoking-
dc.subject.meshUp-Regulation-
dc.titleExpression of DNA repair and metabolic genes in response to a flavonoid-rich diet.en
dc.typeArticleen
dc.identifier.journalThe British Journal of Nutritionen

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