Oxidatively damaged DNA and its repair after experimental exposure to wood smoke in healthy humans.

2.50
Hdl Handle:
http://hdl.handle.net/10146/33832
Title:
Oxidatively damaged DNA and its repair after experimental exposure to wood smoke in healthy humans.
Authors:
Danielsen, Pernille Hogh; Brauner, Elvira Vaclavik; Barregard, Lars; Sallsten, Gerd; Wallin, Maria; Olinski, Ryszard; Rozalski, Rafal; Moller, Peter; Loft, Steffen
Abstract:
Particulate matter from wood smoke may cause health effects through generation of oxidative stress with resulting damage to DNA. We investigated oxidatively damaged DNA and related repair capacity in peripheral blood mononuclear cells (PBMC) and measured the urinary excretion of repair products after controlled short-term exposure of human volunteers to wood smoke. Thirteen healthy adults were exposed first to clean air and then to wood smoke in a chamber during 4h sessions, 1 week apart. Blood samples were taken 3h after exposure and on the following morning, and urine was collected after exposure, from bedtime until the next morning. We measured the levels of DNA strand breaks (SB), oxidized purines as formamidopyrimidine-DNA-glycosylase (FPG) sites and activity of oxoguanine glycosylase 1 (hOGG1) in PBMC by the comet assay, whereas mRNA levels of hOGG1, nucleoside diphosphate linked moiety X-type motif 1 (hNUDT1) and heme oxygenase 1 (hHO1) were determined by real-time RT-PCR. The excretion of 8-oxo-7,8-dihydro-oxoguanine (8-oxoGua) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine was measured by high performance liquid chromatography purification followed by gas chromatography with mass spectrometry. The morning following exposure to wood smoke the PBMC levels of SB were significantly decreased and the mRNA levels of hOGG1 significantly increased. FPG sites, hOGG1 activity, expression of hNUDT1 and hHO1, urinary excretion of 8-oxodG and 8-oxoGua did not change significantly. Our findings support that exposure to wood smoke causes systemic effects, although we could not demonstrate genotoxic effects, possibly explained by enhanced repair and timing of sampling.
Citation:
Mutat. Res. 2008, 642 (1-2):37-42
Journal:
Mutation Research
Issue Date:
3-Jul-2008
URI:
http://hdl.handle.net/10146/33832
DOI:
10.1016/j.mrfmmm.2008.04.001
PubMed ID:
18495177
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2C-4S8TBCC-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=de3e96633626fd29ec3c50d8b64cadb3
Type:
Article
Language:
en
ISSN:
0027-5107
Sponsors:
Pernille Hogh Danielsen, Elvira Vaclavik Brauner, Ryszard Olinski, Rafal Rozalski, Peter Moller and Steffen Loft are partners in ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorDanielsen, Pernille Hogh-
dc.contributor.authorBrauner, Elvira Vaclavik-
dc.contributor.authorBarregard, Lars-
dc.contributor.authorSallsten, Gerd-
dc.contributor.authorWallin, Maria-
dc.contributor.authorOlinski, Ryszard-
dc.contributor.authorRozalski, Rafal-
dc.contributor.authorMoller, Peter-
dc.contributor.authorLoft, Steffen-
dc.date.accessioned2008-08-01T09:23:35Z-
dc.date.available2008-08-01T09:23:35Z-
dc.date.issued2008-07-03-
dc.identifier.citationMutat. Res. 2008, 642 (1-2):37-42en
dc.identifier.issn0027-5107-
dc.identifier.pmid18495177-
dc.identifier.doi10.1016/j.mrfmmm.2008.04.001-
dc.identifier.urihttp://hdl.handle.net/10146/33832-
dc.description.abstractParticulate matter from wood smoke may cause health effects through generation of oxidative stress with resulting damage to DNA. We investigated oxidatively damaged DNA and related repair capacity in peripheral blood mononuclear cells (PBMC) and measured the urinary excretion of repair products after controlled short-term exposure of human volunteers to wood smoke. Thirteen healthy adults were exposed first to clean air and then to wood smoke in a chamber during 4h sessions, 1 week apart. Blood samples were taken 3h after exposure and on the following morning, and urine was collected after exposure, from bedtime until the next morning. We measured the levels of DNA strand breaks (SB), oxidized purines as formamidopyrimidine-DNA-glycosylase (FPG) sites and activity of oxoguanine glycosylase 1 (hOGG1) in PBMC by the comet assay, whereas mRNA levels of hOGG1, nucleoside diphosphate linked moiety X-type motif 1 (hNUDT1) and heme oxygenase 1 (hHO1) were determined by real-time RT-PCR. The excretion of 8-oxo-7,8-dihydro-oxoguanine (8-oxoGua) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine was measured by high performance liquid chromatography purification followed by gas chromatography with mass spectrometry. The morning following exposure to wood smoke the PBMC levels of SB were significantly decreased and the mRNA levels of hOGG1 significantly increased. FPG sites, hOGG1 activity, expression of hNUDT1 and hHO1, urinary excretion of 8-oxodG and 8-oxoGua did not change significantly. Our findings support that exposure to wood smoke causes systemic effects, although we could not demonstrate genotoxic effects, possibly explained by enhanced repair and timing of sampling.en
dc.description.sponsorshipPernille Hogh Danielsen, Elvira Vaclavik Brauner, Ryszard Olinski, Rafal Rozalski, Peter Moller and Steffen Loft are partners in ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2C-4S8TBCC-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=de3e96633626fd29ec3c50d8b64cadb3en
dc.subject8-Oxo-7,8-dihydroguanineen
dc.subject8-Oxo-7,8-dihydro-2′-deoxyguanosineen
dc.subjectDNA damageen
dc.subjectDNA repairen
dc.subjectOxidative stressen
dc.subjectOxoguanine DNA glycosylaseen
dc.subjectStrand breaksen
dc.subjectWood smokeen
dc.subject.meshAir Pollutantsen
dc.subject.meshDNA Damageen
dc.subject.meshDNA Glycosylasesen
dc.subject.meshDNA Repairen
dc.subject.meshHumansen
dc.subject.meshLeukocytes, Mononuclearen
dc.subject.meshOxidative Stressen
dc.subject.meshSmokeen
dc.titleOxidatively damaged DNA and its repair after experimental exposure to wood smoke in healthy humans.en
dc.typeArticleen
dc.identifier.journalMutation Researchen

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