Multiple anticancer effects of damsin and coronopilin isolated from Ambrosia arborescens on cell cultures.

2.50
Hdl Handle:
http://hdl.handle.net/10146/332936
Title:
Multiple anticancer effects of damsin and coronopilin isolated from Ambrosia arborescens on cell cultures.
Authors:
Villagomez, Rodrigo; Rodrigo, Gloria C.; Collado, Isidro G.; Calzado, Marco A.; Muñoz, Eduardo; Åkesson, Björn; Sterner, Olov; Almanza, Giovanna R.; Duan, Rui-Dong
Abstract:
Terpenoids in plants are important sources for drug discovery. In this study, we extracted damsin and coronopilin, two sesquiterpene lactones, from Ambrosia arborescens and examined their anticancer effects on cell cultures. Damsin and coronopilin inhibited cell proliferation, DNA biosynthesis and formation of cytoplasmic DNA histone complexes in Caco-2 cells, with damsin being more potent than coronopilin. Further studies using the luciferase reporter system showed that damsin and coronopilin also inhibited expressions of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription-3 (STAT3), indicating that these sesquiterpenes can interfere with NF-κB and STAT3 pathways. Finally, we examined the effects of two synthetic dibrominated derivatives of damsin, 11α,13-dibromodamsin and 11β,13-dibromodamsin. While bromination appeared to weaken the antiproliferative effects of damsin, the β epimer had strong inhibitory effects on STAT3 activation. In conclusion, the sesquiterpene lactones damsin and coronopilin have inhibitory effects on cell proliferation, DNA biosynthesis and NF-κB and STAT3 pathways, thus being potentially important for discovery of drugs against cancer.
Citation:
Anticancer Res. 2013, 33 (9):3799-3805.
Journal:
Anticancer Research
Issue Date:
Sep-2013
URI:
http://hdl.handle.net/10146/332936
PubMed ID:
24023312
Additional Links:
http://ar.iiarjournals.org/content/33/9/3799.long
Type:
Article
Language:
en
ISSN:
1791-7530
Sponsors:
The study was supported by the Swedish International Development Agency to a joint project on Plant Biodiversity between the Major University of San Andres, La Paz, Bolivia and Lund University, Sweden. Support was also obtained from the EU NoE ECNIS (513943) and its successor ECNIS2.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorVillagomez, Rodrigoen
dc.contributor.authorRodrigo, Gloria C.en
dc.contributor.authorCollado, Isidro G.en
dc.contributor.authorCalzado, Marco A.en
dc.contributor.authorMuñoz, Eduardoen
dc.contributor.authorÅkesson, Björnen
dc.contributor.authorSterner, Oloven
dc.contributor.authorAlmanza, Giovanna R.en
dc.contributor.authorDuan, Rui-Dongen
dc.date.accessioned2014-10-20T12:43:23Z-
dc.date.available2014-10-20T12:43:23Z-
dc.date.issued2013-09-
dc.identifier.citationAnticancer Res. 2013, 33 (9):3799-3805.en
dc.identifier.issn1791-7530-
dc.identifier.pmid24023312-
dc.identifier.urihttp://hdl.handle.net/10146/332936-
dc.description.abstractTerpenoids in plants are important sources for drug discovery. In this study, we extracted damsin and coronopilin, two sesquiterpene lactones, from Ambrosia arborescens and examined their anticancer effects on cell cultures. Damsin and coronopilin inhibited cell proliferation, DNA biosynthesis and formation of cytoplasmic DNA histone complexes in Caco-2 cells, with damsin being more potent than coronopilin. Further studies using the luciferase reporter system showed that damsin and coronopilin also inhibited expressions of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription-3 (STAT3), indicating that these sesquiterpenes can interfere with NF-κB and STAT3 pathways. Finally, we examined the effects of two synthetic dibrominated derivatives of damsin, 11α,13-dibromodamsin and 11β,13-dibromodamsin. While bromination appeared to weaken the antiproliferative effects of damsin, the β epimer had strong inhibitory effects on STAT3 activation. In conclusion, the sesquiterpene lactones damsin and coronopilin have inhibitory effects on cell proliferation, DNA biosynthesis and NF-κB and STAT3 pathways, thus being potentially important for discovery of drugs against cancer.en
dc.description.sponsorshipThe study was supported by the Swedish International Development Agency to a joint project on Plant Biodiversity between the Major University of San Andres, La Paz, Bolivia and Lund University, Sweden. Support was also obtained from the EU NoE ECNIS (513943) and its successor ECNIS2.en
dc.language.isoenen
dc.relation.urlhttp://ar.iiarjournals.org/content/33/9/3799.longen
dc.rightsArchived with thanks to Anticancer researchen
dc.subjectAmbrosia arborescensen
dc.subjectDamsinen
dc.subjectCoronopilinen
dc.subjectSesquiterpenesen
dc.subjectApoptosisen
dc.subjectCell proliferationen
dc.subjectChemoselective brominationen
dc.subjectAnticancer effectsen
dc.subject.meshAmbrosia-
dc.subject.meshAntineoplastic Agents, Phytogenic-
dc.subject.meshAzulenes-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Proliferation-
dc.subject.meshDNA Replication-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshHumans-
dc.subject.meshLactones-
dc.subject.meshNF-kappa B-
dc.subject.meshNeoplasms-
dc.subject.meshSTAT3 Transcription Factor-
dc.subject.meshSesquiterpenes-
dc.titleMultiple anticancer effects of damsin and coronopilin isolated from Ambrosia arborescens on cell cultures.en
dc.typeArticleen
dc.identifier.journalAnticancer Researchen

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