Urinary measurement of 8-OxodG, 8-OxoGua, and 5HMUra: a noninvasive assessment of oxidative damage to DNA.

2.50
Hdl Handle:
http://hdl.handle.net/10146/27994
Title:
Urinary measurement of 8-OxodG, 8-OxoGua, and 5HMUra: a noninvasive assessment of oxidative damage to DNA.
Authors:
Olinski, Ryszard; Rozalski, Rafal; Gackowski, Daniel; Foksinski, Marek; Siomek, Agnieszka; Cooke, Marcus S.
Abstract:
Numerous DNA repair pathways exist to prevent the persistence of damage, and are integral to the maintenance of genome stability, and hence prevention of disease. Excised lesions arising from repair may ultimately appear in the urine where their measurement has been acknowledged to be reflective of overall oxidative stress. The development of reliable assays to measure urinary DNA lesions, such as HPLC prepurification followed by gas chromatography/mass spectrometry, offers the potential to assess whole body oxidative DNA damage. However, some studies suggest a possibility that confounding factors may contribute to urinary levels of 7,8-dihydro-8-oxoguanine (8-oxoGua) and 7,8-dihydro-8-oxo-2 -deoxyguanosine (8-oxodG). This article considers several possible sources of urinary lesions: (a) the repair of oxidatively damaged DNA; (b) a possible dietary influence; and (c) cell death. The authors conclude that data from their laboratories, along with a number of literature reports, form an argument against a contribution from cell death and diet. In the absence of these confounding factors, urinary measurements may be attributed entirely to the repair of DNA damage and suggests their possible use in studying associations between DNA repair and disease.
Citation:
Antioxid. Redox Signal. 2006, 8 (5-6):1011-1019
Journal:
Antioxidants & Redox Signaling
Issue Date:
26-May-2008
URI:
http://hdl.handle.net/10146/27994
DOI:
10.1089/ars.2006.8.1011
PubMed ID:
16771691
Additional Links:
http://www.liebertonline.com/doi/abs/10.1089/ars.2006.8.1011
Type:
Article
Language:
en
ISSN:
1523-0864
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorOlinski, Ryszard-
dc.contributor.authorRozalski, Rafal-
dc.contributor.authorGackowski, Daniel-
dc.contributor.authorFoksinski, Marek-
dc.contributor.authorSiomek, Agnieszka-
dc.contributor.authorCooke, Marcus S.-
dc.date.accessioned2008-05-26T07:38:40Z-
dc.date.available2008-05-26T07:38:40Z-
dc.date.issued2008-05-26T07:38:40Z-
dc.identifier.citationAntioxid. Redox Signal. 2006, 8 (5-6):1011-1019en
dc.identifier.issn1523-0864-
dc.identifier.pmid16771691-
dc.identifier.doi10.1089/ars.2006.8.1011-
dc.identifier.urihttp://hdl.handle.net/10146/27994-
dc.description.abstractNumerous DNA repair pathways exist to prevent the persistence of damage, and are integral to the maintenance of genome stability, and hence prevention of disease. Excised lesions arising from repair may ultimately appear in the urine where their measurement has been acknowledged to be reflective of overall oxidative stress. The development of reliable assays to measure urinary DNA lesions, such as HPLC prepurification followed by gas chromatography/mass spectrometry, offers the potential to assess whole body oxidative DNA damage. However, some studies suggest a possibility that confounding factors may contribute to urinary levels of 7,8-dihydro-8-oxoguanine (8-oxoGua) and 7,8-dihydro-8-oxo-2 -deoxyguanosine (8-oxodG). This article considers several possible sources of urinary lesions: (a) the repair of oxidatively damaged DNA; (b) a possible dietary influence; and (c) cell death. The authors conclude that data from their laboratories, along with a number of literature reports, form an argument against a contribution from cell death and diet. In the absence of these confounding factors, urinary measurements may be attributed entirely to the repair of DNA damage and suggests their possible use in studying associations between DNA repair and disease.en
dc.language.isoenen
dc.relation.urlhttp://www.liebertonline.com/doi/abs/10.1089/ars.2006.8.1011en
dc.subject.meshAnimals-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshDeoxyguanosine-
dc.subject.meshDiet-
dc.subject.meshGuanine-
dc.subject.meshHumans-
dc.subject.meshLife Expectancy-
dc.subject.meshNeoplasms-
dc.subject.meshStatistics as Topic-
dc.subject.meshUracil-
dc.titleUrinary measurement of 8-OxodG, 8-OxoGua, and 5HMUra: a noninvasive assessment of oxidative damage to DNA.en
dc.typeArticleen
dc.identifier.journalAntioxidants & Redox Signalingen

Related articles on PubMed

All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.