The effects of hemodialysis treatment on the level of DNA strand breaks and oxidative DNA lesions measured by the comet assay.

2.50
Hdl Handle:
http://hdl.handle.net/10146/269573
Title:
The effects of hemodialysis treatment on the level of DNA strand breaks and oxidative DNA lesions measured by the comet assay.
Authors:
Ersson, Clara; Odar-Cederlof, Ingegerd; Fehrman-Ekholm, Ingela; Möller, Lennart
Abstract:
Hemodialysis patients have a higher risk for oxidative stress-related complications, such as cardiovascular disease and cancer. The increased level of oxidative stress is due to several factors, e.g., the hemodialysis treatment itself and the uremic state. In the present study, the effects of dialysis treatment on the level of DNA breaks and oxidative DNA lesions in mononuclear cells were measured with the comet assay. Factors possibly affecting DNA damage (reported as % DNA in tail) such as the duration of dialysis, time since last dialysis session, years of dialysis treatment, nutritional status (measured as protein catabolic rate), age, and diabetes were also investigated. The levels of DNA breaks (13.6 ± 4.7 before dialysis) and oxidative DNA lesions (7.9 ± 4.8 before dialysis) were significantly higher in dialysis patients (n = 31) compared to the levels of DNA breaks (5.8 ± 1.1) and oxidative DNA lesions (3.4 ± 1.7) in 10 healthy controls (P < 0.001). A decrease of DNA breaks was observed after dialysis (P = 0.038), and the level of oxidative DNA lesions was higher when the time between two treatment sessions were 68 hours compared to 44 hours (P < 0.001). Older subjects had a higher level of DNA breaks (P = 0.003), a good nutritional status predicted a lower level of DNA breaks (P < 0.001), and the duration of the dialysis session was inversely correlated with oxidative DNA lesions (P = 0.014). Diabetes or years of dialysis treatment did not affect DNA damage. The observations in the present study suggest that accumulation of uremic toxins induce DNA damage. The hemodialysis treatment seems to change the DNA damage.
Citation:
Hemodial. Int. 2013, 17 (3):366-373
Journal:
Hemodialysis international. International Symposium on Home Hemodialysis
Issue Date:
20-Dec-2012
URI:
http://hdl.handle.net/10146/269573
DOI:
10.1111/hdi.12008
PubMed ID:
23279066
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/hdi.12008/abstract;jsessionid=8FE5CAF3316FFE8C5EC60EC3BA7D58E8.d03t01
Type:
Article
Language:
en
ISSN:
1542-4758
Sponsors:
This work was supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract no. 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorErsson, Claraen_GB
dc.contributor.authorOdar-Cederlof, Ingegerden_GB
dc.contributor.authorFehrman-Ekholm, Ingelaen_GB
dc.contributor.authorMöller, Lennarten_GB
dc.date.accessioned2013-02-14T12:35:04Z-
dc.date.available2013-02-14T12:35:04Z-
dc.date.issued2012-12-20-
dc.identifier.citationHemodial. Int. 2013, 17 (3):366-373en_GB
dc.identifier.issn1542-4758-
dc.identifier.pmid23279066-
dc.identifier.doi10.1111/hdi.12008-
dc.identifier.urihttp://hdl.handle.net/10146/269573-
dc.description.abstractHemodialysis patients have a higher risk for oxidative stress-related complications, such as cardiovascular disease and cancer. The increased level of oxidative stress is due to several factors, e.g., the hemodialysis treatment itself and the uremic state. In the present study, the effects of dialysis treatment on the level of DNA breaks and oxidative DNA lesions in mononuclear cells were measured with the comet assay. Factors possibly affecting DNA damage (reported as % DNA in tail) such as the duration of dialysis, time since last dialysis session, years of dialysis treatment, nutritional status (measured as protein catabolic rate), age, and diabetes were also investigated. The levels of DNA breaks (13.6 ± 4.7 before dialysis) and oxidative DNA lesions (7.9 ± 4.8 before dialysis) were significantly higher in dialysis patients (n = 31) compared to the levels of DNA breaks (5.8 ± 1.1) and oxidative DNA lesions (3.4 ± 1.7) in 10 healthy controls (P < 0.001). A decrease of DNA breaks was observed after dialysis (P = 0.038), and the level of oxidative DNA lesions was higher when the time between two treatment sessions were 68 hours compared to 44 hours (P < 0.001). Older subjects had a higher level of DNA breaks (P = 0.003), a good nutritional status predicted a lower level of DNA breaks (P < 0.001), and the duration of the dialysis session was inversely correlated with oxidative DNA lesions (P = 0.014). Diabetes or years of dialysis treatment did not affect DNA damage. The observations in the present study suggest that accumulation of uremic toxins induce DNA damage. The hemodialysis treatment seems to change the DNA damage.en_GB
dc.description.sponsorshipThis work was supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract no. 513943).en_GB
dc.languageENG-
dc.language.isoenen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/hdi.12008/abstract;jsessionid=8FE5CAF3316FFE8C5EC60EC3BA7D58E8.d03t01-
dc.rightsArchived with thanks to Hemodialysis international. International Symposium on Home Hemodialysisen_GB
dc.subjectHemodialysisen_GB
dc.subjectDNA damageen_GB
dc.subjectComet assayen_GB
dc.subjectOxidative Stressen_GB
dc.subjectKidney Diseasesen_GB
dc.titleThe effects of hemodialysis treatment on the level of DNA strand breaks and oxidative DNA lesions measured by the comet assay.en
dc.typeArticleen
dc.identifier.journalHemodialysis international. International Symposium on Home Hemodialysisen_GB

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