Effects of nitrated-polycyclic aromatic hydrocarbons and diesel exhaust particle extracts on cell signalling related to apoptosis: possible implications for their mutagenic and carcinogenic effects.

2.50
Hdl Handle:
http://hdl.handle.net/10146/25253
Title:
Effects of nitrated-polycyclic aromatic hydrocarbons and diesel exhaust particle extracts on cell signalling related to apoptosis: possible implications for their mutagenic and carcinogenic effects.
Authors:
Landvik, Nina E.; Gorria, Morgane; Arlt, Volker M.; Asare, Nana; Solhaug, Anita; Lagadic-Gossmann, Dominique; Holme, Jorn A.
Abstract:
Nitrated-polycyclic aromatic hydrocarbons (nitro-PAHs) and diesel exhaust particle extracts (DEPE) induced apoptosis in Hepa1c1c7 cells with the following potency: 1,3-dinitropyrene (1,3-DNP)>1-nitropyrene (1-NP) >> DEPE >> 1,8-dinitropyrene (1,8-DNP). The compounds induced cyp1a1, and activated various intracellular signalling pathways related to apoptosis. The CYP inhibitor alpha-naphthoflavone strongly reduced 1,3-DNP-induced cell death, whereas cell death induced by 1-NP was rather increased. Toxic 1,3-DNP and 1-NP were found to induce a concentration-dependent lipid peroxidation. 1,3-DNP caused pro-apoptotic events, including increased phosphorylation and accumulation of p53 in the nucleus, cleavage of bid and of caspases 8 and 3, down-regulation of bcl-x(L) and phosphorylation of p38 and JNK MAPK. Furthermore, 1,3-DNP increased the activation of survival signals including phosphorylation of Akt and inactivation (phosphorylation) of pro-apoptotic bad. Although less potent, rather similar effects were observed following exposure to DEPE, compared to 1-NP. The most important finding was that the most mutagenic and carcinogenic compound tested, 1,8-DNP, induced little (if any) cell death, despite the fact that this compound seemed to give the most DNA damage as judged by DNA adduct formation, increased phosphorylation of p53 and accumulation of cells in S-phase. Immunocytochemical studies revealed that the p53 protein did not accumulate into the nucleus suggesting that 1,8-DNP inactivated the pro-apoptotic function of the p53 protein by a non-mutagenic event. These results suggest that after exposure to 1,8-DNP more cells may survive with DNA damage, thereby increasing its mutagenic and carcinogenic potential.
Citation:
Toxicology 2007, 231 (2-3):159-174
Journal:
Toxicology
Issue Date:
7-Mar-2007
URI:
http://hdl.handle.net/10146/25253
DOI:
10.1016/j.tox.2006.12.009
PubMed ID:
17240030
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCN-4MK5GNT-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=56f2434941fd7de2073f2a0e0e082247
Type:
Article
Language:
en
ISSN:
0300-483X
Sponsors:
The study has been supported in part by The Research Council of Norway, by Aurora grant (Egide) and Cancer Research UK; and Volker M. Arlt is a member of the European ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility) Network of Excellence. The technical assistance of Leni Johanne Ekeren and Trine Isaksen is very much appreciated. We also thank Dr. Trine Husøy for help with immunocytochemistry and Dr. Per E. Schwarze for support and critical reading of the manuscript.
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Full metadata record

DC FieldValue Language
dc.contributor.authorLandvik, Nina E.-
dc.contributor.authorGorria, Morgane-
dc.contributor.authorArlt, Volker M.-
dc.contributor.authorAsare, Nana-
dc.contributor.authorSolhaug, Anita-
dc.contributor.authorLagadic-Gossmann, Dominique-
dc.contributor.authorHolme, Jorn A.-
dc.date.accessioned2008-05-09T12:46:45Z-
dc.date.available2008-05-09T12:46:45Z-
dc.date.issued2007-03-07-
dc.identifier.citationToxicology 2007, 231 (2-3):159-174en
dc.identifier.issn0300-483X-
dc.identifier.pmid17240030-
dc.identifier.doi10.1016/j.tox.2006.12.009-
dc.identifier.urihttp://hdl.handle.net/10146/25253-
dc.description.abstractNitrated-polycyclic aromatic hydrocarbons (nitro-PAHs) and diesel exhaust particle extracts (DEPE) induced apoptosis in Hepa1c1c7 cells with the following potency: 1,3-dinitropyrene (1,3-DNP)>1-nitropyrene (1-NP) >> DEPE >> 1,8-dinitropyrene (1,8-DNP). The compounds induced cyp1a1, and activated various intracellular signalling pathways related to apoptosis. The CYP inhibitor alpha-naphthoflavone strongly reduced 1,3-DNP-induced cell death, whereas cell death induced by 1-NP was rather increased. Toxic 1,3-DNP and 1-NP were found to induce a concentration-dependent lipid peroxidation. 1,3-DNP caused pro-apoptotic events, including increased phosphorylation and accumulation of p53 in the nucleus, cleavage of bid and of caspases 8 and 3, down-regulation of bcl-x(L) and phosphorylation of p38 and JNK MAPK. Furthermore, 1,3-DNP increased the activation of survival signals including phosphorylation of Akt and inactivation (phosphorylation) of pro-apoptotic bad. Although less potent, rather similar effects were observed following exposure to DEPE, compared to 1-NP. The most important finding was that the most mutagenic and carcinogenic compound tested, 1,8-DNP, induced little (if any) cell death, despite the fact that this compound seemed to give the most DNA damage as judged by DNA adduct formation, increased phosphorylation of p53 and accumulation of cells in S-phase. Immunocytochemical studies revealed that the p53 protein did not accumulate into the nucleus suggesting that 1,8-DNP inactivated the pro-apoptotic function of the p53 protein by a non-mutagenic event. These results suggest that after exposure to 1,8-DNP more cells may survive with DNA damage, thereby increasing its mutagenic and carcinogenic potential.en
dc.description.sponsorshipThe study has been supported in part by The Research Council of Norway, by Aurora grant (Egide) and Cancer Research UK; and Volker M. Arlt is a member of the European ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility) Network of Excellence. The technical assistance of Leni Johanne Ekeren and Trine Isaksen is very much appreciated. We also thank Dr. Trine Husøy for help with immunocytochemistry and Dr. Per E. Schwarze for support and critical reading of the manuscript.en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCN-4MK5GNT-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=56f2434941fd7de2073f2a0e0e082247en
dc.subjectNitro-polycyclic aromatic hydrocarbonsen
dc.subjectCell signalingen
dc.subjectDNA damageen
dc.subjectp53en
dc.subjectMetabolismen
dc.subjectApoptosisen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCarcinogens, Environmental-
dc.subject.meshCarcinoma, Hepatocellular-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Nucleus-
dc.subject.meshCell Survival-
dc.subject.meshDNA Adducts-
dc.subject.meshDNA Damage-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshHepatocytes-
dc.subject.meshLipid Peroxidation-
dc.subject.meshMice-
dc.subject.meshMutagens-
dc.subject.meshPhosphorylation-
dc.subject.meshPyrenes-
dc.subject.meshQuantitative Structure-Activity Relationship-
dc.subject.meshSignal Transduction-
dc.subject.meshTumor Suppressor Protein p53-
dc.subject.meshVehicle Emissions-
dc.titleEffects of nitrated-polycyclic aromatic hydrocarbons and diesel exhaust particle extracts on cell signalling related to apoptosis: possible implications for their mutagenic and carcinogenic effects.en
dc.typeArticleen
dc.identifier.journalToxicologyen
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