Comparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway.

2.50
Hdl Handle:
http://hdl.handle.net/10146/25216
Title:
Comparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway.
Authors:
Karlsson, Hanna L.; Ljungman, Anders G.; Lindbom, John; Moller, Lennart
Abstract:
The health effects of exposure to airborne particles are of increasing concern in society. In order to protect public health, a clarification of the toxic properties of particles from different sources is of importance. The aim of this study was to investigate and compare the genotoxicity and the ability to induce inflammatory mediators of nine different particle types from wood and pellets combustion, from tire-road wear and collected from an urban street and a subway station. The comet assay was used to assess genotoxicity after exposure of the human lung cell line A549. Inflammatory effects were measured as induction of IL-6, IL-8 and TNF-alpha after exposure of human macrophages. We found that all particles tested caused DNA damage and those from the subway caused more damage than the other particles (p<0.001) likely due to redox-active iron. In contrast, particles collected from an urban street were most potent to induce inflammatory cytokines. Particles from tire-road wear collected using a road simulator were genotoxic and able to induce cytokines. Finally, more effective combustion of wood led to less emission of particles, but those emitted did not show less toxicity in this study.
Citation:
Toxicol. Lett. 2006, 165 (3):203-211
Journal:
Toxicology Letters
Issue Date:
10-Sep-2006
URI:
http://hdl.handle.net/10146/25216
DOI:
10.1016/j.toxlet.2006.04.003
PubMed ID:
16716543
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCR-4JT83T0-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=0ef1c79da4470ab9cd21fb364feb3176
Type:
Article
Language:
en
ISSN:
0378-4274
Sponsors:
We thank Christer Johansson at the City of Stockholm Environment and Health Administration, Mats Gustafsson at the Swedish National Road and Transport Research Institute and Linda Johansson from SP Swedish National Testing and Research Institute for providing the particles. This study was financially supported by the Swedish Environmental Protection Agency. The authors of this paper are partners of the EU Network of Excellence, Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKarlsson, Hanna L.-
dc.contributor.authorLjungman, Anders G.-
dc.contributor.authorLindbom, John-
dc.contributor.authorMoller, Lennart-
dc.date.accessioned2008-05-09T11:06:11Z-
dc.date.available2008-05-09T11:06:11Z-
dc.date.issued2006-09-10-
dc.identifier.citationToxicol. Lett. 2006, 165 (3):203-211en
dc.identifier.issn0378-4274-
dc.identifier.pmid16716543-
dc.identifier.doi10.1016/j.toxlet.2006.04.003-
dc.identifier.urihttp://hdl.handle.net/10146/25216-
dc.description.abstractThe health effects of exposure to airborne particles are of increasing concern in society. In order to protect public health, a clarification of the toxic properties of particles from different sources is of importance. The aim of this study was to investigate and compare the genotoxicity and the ability to induce inflammatory mediators of nine different particle types from wood and pellets combustion, from tire-road wear and collected from an urban street and a subway station. The comet assay was used to assess genotoxicity after exposure of the human lung cell line A549. Inflammatory effects were measured as induction of IL-6, IL-8 and TNF-alpha after exposure of human macrophages. We found that all particles tested caused DNA damage and those from the subway caused more damage than the other particles (p<0.001) likely due to redox-active iron. In contrast, particles collected from an urban street were most potent to induce inflammatory cytokines. Particles from tire-road wear collected using a road simulator were genotoxic and able to induce cytokines. Finally, more effective combustion of wood led to less emission of particles, but those emitted did not show less toxicity in this study.en
dc.description.sponsorshipWe thank Christer Johansson at the City of Stockholm Environment and Health Administration, Mats Gustafsson at the Swedish National Road and Transport Research Institute and Linda Johansson from SP Swedish National Testing and Research Institute for providing the particles. This study was financially supported by the Swedish Environmental Protection Agency. The authors of this paper are partners of the EU Network of Excellence, Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS).en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCR-4JT83T0-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=0ef1c79da4470ab9cd21fb364feb3176en
dc.subjectParticulate matteren
dc.subjectSubwayen
dc.subjectComet assayen
dc.subjectGenotoxicityen
dc.subjectInflammationen
dc.subjectCytokinesen
dc.subject.meshAir Pollutants-
dc.subject.meshCell Line-
dc.subject.meshEpithelial Cells-
dc.subject.meshFires-
dc.subject.meshHumans-
dc.subject.meshInflammation-
dc.subject.meshInterleukin-6-
dc.subject.meshInterleukin-8-
dc.subject.meshMacrophages-
dc.subject.meshMotor Vehicles-
dc.subject.meshMutagens-
dc.subject.meshRailroads-
dc.subject.meshRespiratory Mucosa-
dc.subject.meshSmoke-
dc.subject.meshTumor Necrosis Factor-alpha-
dc.subject.meshWood-
dc.titleComparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway.en
dc.typeArticleen
dc.identifier.journalToxicology Lettersen

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