DNA oxidative damage and strand breaks in young healthy individuals: a gender difference and the role of life style factors.

2.50
Hdl Handle:
http://hdl.handle.net/10146/25213
Title:
DNA oxidative damage and strand breaks in young healthy individuals: a gender difference and the role of life style factors.
Authors:
Hofer, Tim; Karlsson, Hanna L.; Moller, Lennart
Abstract:
The aim of this study was to analyze background levels of DNA damage in young (19-31 years) non-smoking individuals and to correlate damage to gender and life style. DNA single strand breaks (SSB) and alkali labile sites (ALS) were measured in 99 subjects living in Stockholm, Sweden. Further, oxidative DNA damage was analyzed using the DNA repair glycosylase FPG as well as HPLC-ECD for specific analysis of 8-oxo-7,8-dihydro-2'deoxyguanosine (8-oxodG). We found that males had higher (P < 0.001) levels of SSB + ALS than females, but no difference was seen for oxidative lesions. There was no correlation between FPG sites and 8-oxodG. For females, there was a positive correlation between FPG levels and body mass index and a negative correlation between SSB + ALS and fruit intake. We conclude that the background level of oxidative DNA damage, analyzed with improved methods, is low and that gender, fruit intake and BMI can affect DNA damage.
Citation:
Free Radic. Res. 2006, 40 (7):707-714
Journal:
Free Radical Research
Issue Date:
Jul-2006
URI:
http://hdl.handle.net/10146/25213
PubMed ID:
16983997
Additional Links:
http://www.informaworld.com/smpp/title~content=t713642632
Type:
Article
Language:
en
ISSN:
1071-5762
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorHofer, Tim-
dc.contributor.authorKarlsson, Hanna L.-
dc.contributor.authorMoller, Lennart-
dc.date.accessioned2008-05-09T10:49:26Z-
dc.date.available2008-05-09T10:49:26Z-
dc.date.issued2006-07-
dc.identifier.citationFree Radic. Res. 2006, 40 (7):707-714en
dc.identifier.issn1071-5762-
dc.identifier.pmid16983997-
dc.identifier.urihttp://hdl.handle.net/10146/25213-
dc.description.abstractThe aim of this study was to analyze background levels of DNA damage in young (19-31 years) non-smoking individuals and to correlate damage to gender and life style. DNA single strand breaks (SSB) and alkali labile sites (ALS) were measured in 99 subjects living in Stockholm, Sweden. Further, oxidative DNA damage was analyzed using the DNA repair glycosylase FPG as well as HPLC-ECD for specific analysis of 8-oxo-7,8-dihydro-2'deoxyguanosine (8-oxodG). We found that males had higher (P < 0.001) levels of SSB + ALS than females, but no difference was seen for oxidative lesions. There was no correlation between FPG sites and 8-oxodG. For females, there was a positive correlation between FPG levels and body mass index and a negative correlation between SSB + ALS and fruit intake. We conclude that the background level of oxidative DNA damage, analyzed with improved methods, is low and that gender, fruit intake and BMI can affect DNA damage.en
dc.language.isoenen
dc.relation.urlhttp://www.informaworld.com/smpp/title~content=t713642632en
dc.subjectComet assayen
dc.subjectDNA damageen
dc.subjectenvironmenten
dc.subjectoxidative stressen
dc.subjectgenderen
dc.subjectlymphocyteen
dc.subject.meshAdult-
dc.subject.meshBody Mass Index-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshDNA-Formamidopyrimidine Glycosylase-
dc.subject.meshDeoxyguanosine-
dc.subject.meshDiet-
dc.subject.meshEscherichia coli Proteins-
dc.subject.meshFemale-
dc.subject.meshFruit-
dc.subject.meshHumans-
dc.subject.meshLife Style-
dc.subject.meshLymphocytes-
dc.subject.meshMale-
dc.subject.meshMass Spectrometry-
dc.subject.meshOxidation-Reduction-
dc.subject.meshSex Characteristics-
dc.subject.meshSmoking-
dc.subject.meshStress-
dc.subject.meshSweden-
dc.titleDNA oxidative damage and strand breaks in young healthy individuals: a gender difference and the role of life style factors.en
dc.typeArticleen
dc.identifier.journalFree Radical Researchen

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