Evidence for attenuated cellular 8-oxo-7,8-dihydro-2'-deoxyguanosine removal in cancer patients.

2.50
Hdl Handle:
http://hdl.handle.net/10146/24133
Title:
Evidence for attenuated cellular 8-oxo-7,8-dihydro-2'-deoxyguanosine removal in cancer patients.
Authors:
Cooke, Marcus S.; Rozalski, Rafal; Dove, Rosamund; Gackowski, Daniel; Siomek, Agnieszka; Evans, Mark D.; Olinski, Ryszard
Abstract:
Measurement of the products of oxidatively damaged DNA in urine is a frequently used means by which oxidative stress may be assessed non-invasively. We believe that urinary DNA lesions, in addition to being biomarkers of oxidative stress, can potentially provide more specific information, for example, a reflection of repair activity. We used high-performance liquid chromatography prepurification, with gas chromatography-mass spectrometry (LC-GC-MS) and ELISA to the analysis of a number of oxidative [e.g., 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-guanine, 5-(hydroxymethyl)uracil], non-oxidative (cyclobutane thymine dimers) and oligomeric DNA products in urine. We analysed spot urine samples from 20 healthy subjects, and 20 age- and sex-matched cancer patients. Mononuclear cell DNA 8-oxodG levels were assessed by LC-EC. The data support our proposal that urinary DNA lesion products are predominantly derived from DNA repair. Furthermore, analysis of DNA and urinary 8-oxodG in cancer patients and controls suggested reduced repair activity towards this lesion marker in these patients.
Citation:
Biol. Chem. 2006, 387 (4):393-400
Journal:
Biological Chemistry
Issue Date:
Apr-2006
URI:
http://hdl.handle.net/10146/24133
DOI:
10.1515/BC.2006.053
PubMed ID:
16606337
Additional Links:
http://www.atypon-link.com/doi/abs/10.1515/BC.2006.053
Type:
Article
Language:
en
ISSN:
1431-6730
Sponsors:
MSC and MDE would like to acknowledge financial support from the UK Food Standards Agency, and Arthritis Research Campaign. RO acknowledges partial financial support by a grant from the Committee for Science Research (No. PBZ-KBN-091/ PO5/55). The authors also acknowledge financial support from the EU NoE ‘ECNIS’ grant FOOD-CT-2005-513943. The authors acknowledge the assistance of the Department of Chemical Pathology, Leicester Royal Infirmary, UK, for performing the analysis of urinary uric acid.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorCooke, Marcus S.-
dc.contributor.authorRozalski, Rafal-
dc.contributor.authorDove, Rosamund-
dc.contributor.authorGackowski, Daniel-
dc.contributor.authorSiomek, Agnieszka-
dc.contributor.authorEvans, Mark D.-
dc.contributor.authorOlinski, Ryszard-
dc.date.accessioned2008-04-24T11:17:44Z-
dc.date.available2008-04-24T11:17:44Z-
dc.date.issued2006-04-
dc.identifier.citationBiol. Chem. 2006, 387 (4):393-400en
dc.identifier.issn1431-6730-
dc.identifier.pmid16606337-
dc.identifier.doi10.1515/BC.2006.053-
dc.identifier.urihttp://hdl.handle.net/10146/24133-
dc.description.abstractMeasurement of the products of oxidatively damaged DNA in urine is a frequently used means by which oxidative stress may be assessed non-invasively. We believe that urinary DNA lesions, in addition to being biomarkers of oxidative stress, can potentially provide more specific information, for example, a reflection of repair activity. We used high-performance liquid chromatography prepurification, with gas chromatography-mass spectrometry (LC-GC-MS) and ELISA to the analysis of a number of oxidative [e.g., 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-guanine, 5-(hydroxymethyl)uracil], non-oxidative (cyclobutane thymine dimers) and oligomeric DNA products in urine. We analysed spot urine samples from 20 healthy subjects, and 20 age- and sex-matched cancer patients. Mononuclear cell DNA 8-oxodG levels were assessed by LC-EC. The data support our proposal that urinary DNA lesion products are predominantly derived from DNA repair. Furthermore, analysis of DNA and urinary 8-oxodG in cancer patients and controls suggested reduced repair activity towards this lesion marker in these patients.en
dc.description.sponsorshipMSC and MDE would like to acknowledge financial support from the UK Food Standards Agency, and Arthritis Research Campaign. RO acknowledges partial financial support by a grant from the Committee for Science Research (No. PBZ-KBN-091/ PO5/55). The authors also acknowledge financial support from the EU NoE ‘ECNIS’ grant FOOD-CT-2005-513943. The authors acknowledge the assistance of the Department of Chemical Pathology, Leicester Royal Infirmary, UK, for performing the analysis of urinary uric acid.en
dc.language.isoenen
dc.relation.urlhttp://www.atypon-link.com/doi/abs/10.1515/BC.2006.053en
dc.subjectDNAen
dc.subjectDNA repairen
dc.subject8-oxo-7,8-dihydro-2′-deoxyguanosineen
dc.subject8-oxo-7,8-dihydro-guanineen
dc.subjecturineen
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshCase-Control Studies-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshColorectal Neoplasms-
dc.subject.meshDNA-
dc.subject.meshDeoxyguanosine-
dc.subject.meshFemale-
dc.subject.meshGas Chromatography-Mass Spectrometry-
dc.subject.meshHumans-
dc.subject.meshLeukocytes-
dc.subject.meshLung Neoplasms-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshTumor Markers, Biological-
dc.titleEvidence for attenuated cellular 8-oxo-7,8-dihydro-2'-deoxyguanosine removal in cancer patients.en
dc.typeArticleen
dc.identifier.journalBiological Chemistryen

Related articles on PubMed

All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.