Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.

2.50
Hdl Handle:
http://hdl.handle.net/10146/24116
Title:
Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.
Authors:
Bruzelius, K.; Hoac, T.; Sundler, R.; Onning, G.; Akesson, B.
Abstract:
To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation.
Citation:
J. Dairy Sci. 2007, 90 (2):918-927
Journal:
Journal of Dairy Science
Issue Date:
Feb-2007
URI:
http://hdl.handle.net/10146/24116
PubMed ID:
17235168
Additional Links:
http://jds.fass.org/cgi/content/full/90/2/918
Type:
Article
Language:
en
ISSN:
1525-3198
Sponsors:
Biomedical Nutrition is a member of the EU NoE The European Nutrigenomics Organisation (NuGO, no. 505944) and the NoE Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorBruzelius, K.-
dc.contributor.authorHoac, T.-
dc.contributor.authorSundler, R.-
dc.contributor.authorOnning, G.-
dc.contributor.authorAkesson, B.-
dc.date.accessioned2008-04-24T10:59:10Z-
dc.date.available2008-04-24T10:59:10Z-
dc.date.issued2007-02-
dc.identifier.citationJ. Dairy Sci. 2007, 90 (2):918-927en
dc.identifier.issn1525-3198-
dc.identifier.pmid17235168-
dc.identifier.urihttp://hdl.handle.net/10146/24116-
dc.description.abstractTo elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation.en
dc.description.sponsorshipBiomedical Nutrition is a member of the EU NoE The European Nutrigenomics Organisation (NuGO, no. 505944) and the NoE Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.en
dc.language.isoenen
dc.relation.urlhttp://jds.fass.org/cgi/content/full/90/2/918en
dc.subjectmammary tissueen
dc.subjectselenoproteinen
dc.subjectglutathione peroxidaseen
dc.subjectthioredoxin reductaseen
dc.subject.meshAnimals-
dc.subject.meshBlotting, Northern-
dc.subject.meshCattle-
dc.subject.meshFemale-
dc.subject.meshGlutathione Peroxidase-
dc.subject.meshMammary Glands, Animal-
dc.subject.meshNucleic Acid Hybridization-
dc.subject.meshRNA, Messenger-
dc.subject.meshSelenium-
dc.subject.meshSelenoprotein P-
dc.subject.meshSelenoproteins-
dc.subject.meshSolubility-
dc.subject.meshThioredoxin Reductase 1-
dc.subject.meshThioredoxin-Disulfide Reductase-
dc.titleOccurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.en
dc.typeArticleen
dc.identifier.journalJournal of Dairy Scienceen

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