Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods.

2.50
Hdl Handle:
http://hdl.handle.net/10146/234451
Title:
Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods.
Authors:
Nagy, Eszter; Johansson, Clara; Zeisig, Magnus; Moller, Lennart
Abstract:
The air pollutant 3-nitrobenzanthrone (3-NBA), emitted in diesel exhaust, is a potent mutagen and genotoxin. 3-NBA can isomerise to 2-nitrobenzanthrone (2-NBA), which can become more than 70-fold higher in concentration in ambient air. In this study, three independent methods have been employed to evaluate the oxidative stress and genotoxicity of 2-NBA compared to 3-NBA in the human A549 lung cell line. HPLC-EC/UV was applied for measurements of oxidative damage in the form of 8-oxo-2'-deoxyguanosine (8-oxodG), (32)P-HPLC for measurements of lipophilic DNA-adducts, and the Comet assay to measure a variety of DNA lesions, including oxidative stress. No significant oxidative damage from either isomer was found regarding formation of 8-oxodG analysed using HPLC-EC/UV. However, the Comet assay (with FPG-treatment), which is more sensitive and detects more types of damages compared to HPLC-EC/UV, showed a significant effect from both 3-NBA and 2-NBA. (32)P-HPLC revealed a strong DNA-adduct formation from both 3-NBA and 2-NBA, and also a significant difference between both isomers compared to negative control. These results clearly show that 2-NBA has a genotoxic potential. Even if the DNA-adduct forming capacity and the amount of DNA lesions measured with the (32)P-HPLC and Comet assay is about one third of 3-NBA, the high abundance of 2-NBA in ambient air calls for further investigation and evaluation of its health hazard.
Citation:
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2005, 827(1):94-103
Journal:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Issue Date:
15-Nov-2005
URI:
http://hdl.handle.net/10146/234451
DOI:
10.1016/j.jchromb.2005.03.014
PubMed ID:
16260381
Additional Links:
http://www.sciencedirect.com/science/article/pii/S1570023205002126
Type:
Article
Language:
en
ISSN:
1570-0232
Sponsors:
This project was supported by the Swedish Environmental Protection Agency. The authors of this paper are partners of the EU network of ECNIS (European Cancer Risk, Nutrition and Individual Susceptibility).
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorNagy, Eszteren_GB
dc.contributor.authorJohansson, Claraen_GB
dc.contributor.authorZeisig, Magnusen_GB
dc.contributor.authorMoller, Lennarten_GB
dc.date.accessioned2012-07-19T07:34:22Z-
dc.date.available2012-07-19T07:34:22Z-
dc.date.issued2005-11-15-
dc.identifier.citationJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2005, 827(1):94-103en_GB
dc.identifier.issn1570-0232-
dc.identifier.pmid16260381-
dc.identifier.doi10.1016/j.jchromb.2005.03.014-
dc.identifier.urihttp://hdl.handle.net/10146/234451-
dc.description.abstractThe air pollutant 3-nitrobenzanthrone (3-NBA), emitted in diesel exhaust, is a potent mutagen and genotoxin. 3-NBA can isomerise to 2-nitrobenzanthrone (2-NBA), which can become more than 70-fold higher in concentration in ambient air. In this study, three independent methods have been employed to evaluate the oxidative stress and genotoxicity of 2-NBA compared to 3-NBA in the human A549 lung cell line. HPLC-EC/UV was applied for measurements of oxidative damage in the form of 8-oxo-2'-deoxyguanosine (8-oxodG), (32)P-HPLC for measurements of lipophilic DNA-adducts, and the Comet assay to measure a variety of DNA lesions, including oxidative stress. No significant oxidative damage from either isomer was found regarding formation of 8-oxodG analysed using HPLC-EC/UV. However, the Comet assay (with FPG-treatment), which is more sensitive and detects more types of damages compared to HPLC-EC/UV, showed a significant effect from both 3-NBA and 2-NBA. (32)P-HPLC revealed a strong DNA-adduct formation from both 3-NBA and 2-NBA, and also a significant difference between both isomers compared to negative control. These results clearly show that 2-NBA has a genotoxic potential. Even if the DNA-adduct forming capacity and the amount of DNA lesions measured with the (32)P-HPLC and Comet assay is about one third of 3-NBA, the high abundance of 2-NBA in ambient air calls for further investigation and evaluation of its health hazard.en_GB
dc.description.sponsorshipThis project was supported by the Swedish Environmental Protection Agency. The authors of this paper are partners of the EU network of ECNIS (European Cancer Risk, Nutrition and Individual Susceptibility).en_GB
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1570023205002126en_GB
dc.rightsArchived with thanks to Journal of chromatography. B, Analytical technologies in the biomedical and life sciencesen_GB
dc.subjectAir pollutanten_GB
dc.subjectBenz(a)anthracenesen_GB
dc.subjectToxicityen_GB
dc.subjectHumansen_GB
dc.subjectLungen_GB
dc.subjectDNA damageen_GB
dc.subjectOxidative stressen_GB
dc.subjectComet assayen_GB
dc.subjectChromatography, High Pressure Liquiden_GB
dc.subject.meshAir Pollutants-
dc.subject.meshBenz(a)Anthracenes-
dc.subject.meshCell Line-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshDeoxyguanosine-
dc.subject.meshHumans-
dc.subject.meshLung-
dc.subject.meshOxidative Stress-
dc.subject.meshPhosphorus Radioisotopes-
dc.titleOxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods.en
dc.typeArticleen
dc.identifier.journalJournal of chromatography. B, Analytical technologies in the biomedical and life sciencesen_GB
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