Stability and reproducibility of simultaneously detected plasma and serum cytokine levels in asymptomatic subjects.

2.50
Hdl Handle:
http://hdl.handle.net/10146/234211
Title:
Stability and reproducibility of simultaneously detected plasma and serum cytokine levels in asymptomatic subjects.
Authors:
Saberi Hosnijeh, Fatemeh; Krop, Esmeralda J. M.; Portengen, Lutzen; Rabkin, Charles S.; Linseisen, Jakob; Vineis, Paolo; Vermeulen, Roel
Abstract:
Blood levels of cyto- and chemokines might reflect immune deregulations which might be related to lymphomagenesis. Potential utility of stored blood samples of a prospective cohort was evaluated by the effect of different blood sample types and freeze-thaw cycles on analyte levels. Bead-based immunoassays were performed on two fresh samples (serum, citrate and heparin plasma) of 10 asymptomatic adults collected 14 days apart and on aliquots of the first samples which were put through one to three freeze-thaw cycles to measure 11 cytokines, four chemokines and two adhesion molecules. Median coefficients of variation (CVs) of the measured analytes were 20%, 24% and 32% in serum, citrate and heparin plasma, respectively. Strong correlations (rank correlation coefficient 0.74-0.98) were observed between sample types, although small differences in analyte levels were observed for most analytes. Freeze-thaw cycles did not markedly change analyte levels. Our study supports the use of this assay among asymptomatic subjects in epidemiological studies.
Citation:
Biomarkers 2010, 15 (2):140-148
Journal:
Biomarkers : Biochemical indicators of exposure, response, and susceptibility to chemicals
Issue Date:
Mar-2010
URI:
http://hdl.handle.net/10146/234211
DOI:
10.3109/13547500903340570
PubMed ID:
19848603
Additional Links:
http://informahealthcare.com/doi/abs/10.3109/13547500903340570
Type:
Article
Language:
en
ISSN:
1366-5804
Sponsors:
This work was supported by the ECNIS Network of Excellence (Environmental Cancer Risk, Nutrition and Individual Susceptibility), operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety (FOOD-CT-2005- 513943). The first author also acknowledges the Iranian Ministry of Health and Medical Education for support of a PhD programme at Utrecht University.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorSaberi Hosnijeh, Fatemehen_GB
dc.contributor.authorKrop, Esmeralda J. M.en_GB
dc.contributor.authorPortengen, Lutzenen_GB
dc.contributor.authorRabkin, Charles S.en_GB
dc.contributor.authorLinseisen, Jakoben_GB
dc.contributor.authorVineis, Paoloen_GB
dc.contributor.authorVermeulen, Roelen_GB
dc.date.accessioned2012-07-17T19:49:00Z-
dc.date.available2012-07-17T19:49:00Z-
dc.date.issued2010-03-
dc.identifier.citationBiomarkers 2010, 15 (2):140-148en_GB
dc.identifier.issn1366-5804-
dc.identifier.pmid19848603-
dc.identifier.doi10.3109/13547500903340570-
dc.identifier.urihttp://hdl.handle.net/10146/234211-
dc.description.abstractBlood levels of cyto- and chemokines might reflect immune deregulations which might be related to lymphomagenesis. Potential utility of stored blood samples of a prospective cohort was evaluated by the effect of different blood sample types and freeze-thaw cycles on analyte levels. Bead-based immunoassays were performed on two fresh samples (serum, citrate and heparin plasma) of 10 asymptomatic adults collected 14 days apart and on aliquots of the first samples which were put through one to three freeze-thaw cycles to measure 11 cytokines, four chemokines and two adhesion molecules. Median coefficients of variation (CVs) of the measured analytes were 20%, 24% and 32% in serum, citrate and heparin plasma, respectively. Strong correlations (rank correlation coefficient 0.74-0.98) were observed between sample types, although small differences in analyte levels were observed for most analytes. Freeze-thaw cycles did not markedly change analyte levels. Our study supports the use of this assay among asymptomatic subjects in epidemiological studies.en_GB
dc.description.sponsorshipThis work was supported by the ECNIS Network of Excellence (Environmental Cancer Risk, Nutrition and Individual Susceptibility), operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety (FOOD-CT-2005- 513943). The first author also acknowledges the Iranian Ministry of Health and Medical Education for support of a PhD programme at Utrecht University.en_GB
dc.language.isoenen
dc.relation.urlhttp://informahealthcare.com/doi/abs/10.3109/13547500903340570en_GB
dc.rightsArchived with thanks to Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicalsen_GB
dc.subjectCytokineen_GB
dc.subjectDrug stabilityen_GB
dc.subjectAnticoagulantsen_GB
dc.subjectFreezingen_GB
dc.subjectReproducibilityen_GB
dc.subjectSerumen_GB
dc.subjectHumansen_GB
dc.subject.meshAdult-
dc.subject.meshAnticoagulants-
dc.subject.meshBlood Specimen Collection-
dc.subject.meshChemokines-
dc.subject.meshCitric Acid-
dc.subject.meshCryopreservation-
dc.subject.meshCytokines-
dc.subject.meshDrug Stability-
dc.subject.meshFreezing-
dc.subject.meshHeparin-
dc.subject.meshHumans-
dc.subject.meshPlasma-
dc.subject.meshReproducibility of Results-
dc.subject.meshSerum-
dc.subject.meshSpecimen Handling-
dc.titleStability and reproducibility of simultaneously detected plasma and serum cytokine levels in asymptomatic subjects.en
dc.typeArticleen
dc.identifier.journalBiomarkers : Biochemical indicators of exposure, response, and susceptibility to chemicalsen_GB

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