DNA repair gene expression level in peripheral blood and tumour tissue from non-small cell lung cancer and head and neck squamous cell cancer patients.

2.50
Hdl Handle:
http://hdl.handle.net/10146/232091
Title:
DNA repair gene expression level in peripheral blood and tumour tissue from non-small cell lung cancer and head and neck squamous cell cancer patients.
Authors:
Schena, Marina; Guarrera, Simonetta; Buffoni, Lucio; Salvadori, Angelica; Voglino, Floriana; Allione, Alessandra; Pecorari, Giancarlo; Ruffini, Enrico; Garzino-Demo, Paolo; Bustreo, Sara; Consito, Lorena; Bironzo, Paolo; Matullo, Giuseppe
Abstract:
The nucleotide excision repair pathway is crucial for cellular DNA integrity and the ERCC1 helicase is also potentially involved in resistance to platinum-based chemotherapy, and high levels of ERCC1 mRNA in tumours have been associated with cisplatin resistance in different human cancers. The aim of this work was to investigate the correlation between DNA repair gene expression levels in tumour tissue, normal tissue and peripheral blood samples from patients with two common human cancers, non-small cell lung cancer (NSCLC) and squamous cell carcinoma of the head and neck (HNSCC), to test if blood gene expression could be a proxy for tumour tissue gene expression to predict response to platinum-based chemotherapy.; Using RT-qPCR we determined ERCC1, ERCC2, ERCC4, XPA, XPC, XRCC1, XRCC3, APEX, OGG1, MGMT mRNA levels in fresh NSCLC, normal lung and HNSCC tissue, as well as blood, from NSCLC and HNSCC patients who were treated surgically.; Target gene expression in NSCLC and HNSCC tissue was higher than in blood. A statistically significant correlation (p<0.05) was found between target gene mRNA expression in tumour tissue and blood, in particular ERCC1, MGMT, XPC, XRCC1 and XRCC3 in NSCLC and APEX, ERCC1, ERCC2, ERCC4, XRCC1 and XRCC3 in HNSCC.; The existence of a significant correlation between blood and tumour tissue expression of some genes of clinical interest, such as ERCC1 in NSCLC and HNSCC, could allow the introduction in clinical practice of a simple test that would measure mRNA levels of DNA repair genes in peripheral blood samples instead of tissue samples to determine prognostic and predictive factors in NSCLC and HNSCC patients.
Citation:
DNA Repair (Amst.) 2012, 11 (4):374-380
Journal:
DNA Repair
Issue Date:
1-Apr-2012
URI:
http://hdl.handle.net/10146/232091
DOI:
10.1016/j.dnarep.2012.01.003
PubMed ID:
22284908
Additional Links:
http://www.sciencedirect.com/science/article/pii/S1568786412000171
Type:
Article
Language:
en
ISSN:
1568-7856
Sponsors:
This work was supported by a grant of the Associazione Italiana per la Ricerca sul Cancro (Italy; G.M.), of the Progetto Integrato Oncologia, Regione Toscana – Ministero della Salute “Identification of population risk profiles as an approach to cancer prevention” and of the Environmental Cancer Risk Nutrition and Individual Susceptibility project (G.M.), a network of excellence operating within the European Union Sixth Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No. 513943). A partial funding for this project has also been received from the Compagnia di San Paolo (Turin, Italy; G.M.) and by Progetto Ricerca Sanitaria Finalizzata Regione Piemonte (G.M., M.S.).
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Full metadata record

DC FieldValue Language
dc.contributor.authorSchena, Marinaen_GB
dc.contributor.authorGuarrera, Simonettaen_GB
dc.contributor.authorBuffoni, Lucioen_GB
dc.contributor.authorSalvadori, Angelicaen_GB
dc.contributor.authorVoglino, Florianaen_GB
dc.contributor.authorAllione, Alessandraen_GB
dc.contributor.authorPecorari, Giancarloen_GB
dc.contributor.authorRuffini, Enricoen_GB
dc.contributor.authorGarzino-Demo, Paoloen_GB
dc.contributor.authorBustreo, Saraen_GB
dc.contributor.authorConsito, Lorenaen_GB
dc.contributor.authorBironzo, Paoloen_GB
dc.contributor.authorMatullo, Giuseppeen_GB
dc.date.accessioned2012-07-04T11:05:46Z-
dc.date.available2012-07-04T11:05:46Z-
dc.date.issued2012-04-01-
dc.identifier.citationDNA Repair (Amst.) 2012, 11 (4):374-380en_GB
dc.identifier.issn1568-7856-
dc.identifier.pmid22284908-
dc.identifier.doi10.1016/j.dnarep.2012.01.003-
dc.identifier.urihttp://hdl.handle.net/10146/232091-
dc.description.abstractThe nucleotide excision repair pathway is crucial for cellular DNA integrity and the ERCC1 helicase is also potentially involved in resistance to platinum-based chemotherapy, and high levels of ERCC1 mRNA in tumours have been associated with cisplatin resistance in different human cancers. The aim of this work was to investigate the correlation between DNA repair gene expression levels in tumour tissue, normal tissue and peripheral blood samples from patients with two common human cancers, non-small cell lung cancer (NSCLC) and squamous cell carcinoma of the head and neck (HNSCC), to test if blood gene expression could be a proxy for tumour tissue gene expression to predict response to platinum-based chemotherapy.en_GB
dc.description.abstractUsing RT-qPCR we determined ERCC1, ERCC2, ERCC4, XPA, XPC, XRCC1, XRCC3, APEX, OGG1, MGMT mRNA levels in fresh NSCLC, normal lung and HNSCC tissue, as well as blood, from NSCLC and HNSCC patients who were treated surgically.en_GB
dc.description.abstractTarget gene expression in NSCLC and HNSCC tissue was higher than in blood. A statistically significant correlation (p<0.05) was found between target gene mRNA expression in tumour tissue and blood, in particular ERCC1, MGMT, XPC, XRCC1 and XRCC3 in NSCLC and APEX, ERCC1, ERCC2, ERCC4, XRCC1 and XRCC3 in HNSCC.en_GB
dc.description.abstractThe existence of a significant correlation between blood and tumour tissue expression of some genes of clinical interest, such as ERCC1 in NSCLC and HNSCC, could allow the introduction in clinical practice of a simple test that would measure mRNA levels of DNA repair genes in peripheral blood samples instead of tissue samples to determine prognostic and predictive factors in NSCLC and HNSCC patients.en_GB
dc.description.sponsorshipThis work was supported by a grant of the Associazione Italiana per la Ricerca sul Cancro (Italy; G.M.), of the Progetto Integrato Oncologia, Regione Toscana – Ministero della Salute “Identification of population risk profiles as an approach to cancer prevention” and of the Environmental Cancer Risk Nutrition and Individual Susceptibility project (G.M.), a network of excellence operating within the European Union Sixth Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No. 513943). A partial funding for this project has also been received from the Compagnia di San Paolo (Turin, Italy; G.M.) and by Progetto Ricerca Sanitaria Finalizzata Regione Piemonte (G.M., M.S.).en_GB
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1568786412000171en_GB
dc.rightsArchived with thanks to DNA repairen_GB
dc.subjectLung cancersen_GB
dc.subjectNon-small cell lung canceren_GB
dc.subjectHead and neck squamous cell canceren_GB
dc.subjectGene Expressionen_GB
dc.subjectDNA repairen_GB
dc.subjectPeripheral blooden_GB
dc.subjectTumour tissueen_GB
dc.subjectHumanen_GB
dc.titleDNA repair gene expression level in peripheral blood and tumour tissue from non-small cell lung cancer and head and neck squamous cell cancer patients.en
dc.typeArticleen
dc.identifier.journalDNA Repairen_GB

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