Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.

2.50
Hdl Handle:
http://hdl.handle.net/10146/232051
Title:
Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.
Authors:
Bruzelius, Katharina; Sundler, Roger; Pagmantidis, Vasileios; Akesson, Bjorn
Abstract:
Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary.
Citation:
J. Trace Elem. Med. Biol. 2010, 24 (4):251-256
Journal:
Journal of Trace Elements in Medicine and Biology
Issue Date:
Oct-2010
URI:
http://hdl.handle.net/10146/232051
DOI:
10.1016/j.jtemb.2010.08.001
PubMed ID:
20943360
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0946672X10000805
Type:
Article
Language:
en
ISSN:
1878-3252
Sponsors:
These studies were supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), the Lund University Hospital, and the Påhlsson Foundation. Biomedical Nutrition is a member of the EU NoE, The European Nutrigenomics Organisation (NuGO, no. 505944) and the NoE, Environmental Carcinogenesis, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorBruzelius, Katharinaen_GB
dc.contributor.authorSundler, Rogeren_GB
dc.contributor.authorPagmantidis, Vasileiosen_GB
dc.contributor.authorAkesson, Bjornen_GB
dc.date.accessioned2012-07-04T08:24:07Z-
dc.date.available2012-07-04T08:24:07Z-
dc.date.issued2010-10-
dc.identifier.citationJ. Trace Elem. Med. Biol. 2010, 24 (4):251-256en_GB
dc.identifier.issn1878-3252-
dc.identifier.pmid20943360-
dc.identifier.doi10.1016/j.jtemb.2010.08.001-
dc.identifier.urihttp://hdl.handle.net/10146/232051-
dc.description.abstractSelenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary.en_GB
dc.description.sponsorshipThese studies were supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), the Lund University Hospital, and the Påhlsson Foundation. Biomedical Nutrition is a member of the EU NoE, The European Nutrigenomics Organisation (NuGO, no. 505944) and the NoE, Environmental Carcinogenesis, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.en_GB
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0946672X10000805en_GB
dc.rightsArchived with thanks to Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)en_GB
dc.subjectSelenoproteinsen_GB
dc.subjectRNA, Messengeren_GB
dc.subjectGene Expressionen_GB
dc.subjectHormonesen_GB
dc.subjectDrug effectsen_GB
dc.subjectAnimalsen_GB
dc.subjectCell Lineen_GB
dc.subjectMammary glanden_GB
dc.subjectProlactinen_GB
dc.subjectInsulinen_GB
dc.subject.meshAnimals-
dc.subject.meshCattle-
dc.subject.meshCell Line-
dc.subject.meshGene Expression-
dc.subject.meshHormones-
dc.subject.meshHydrocortisone-
dc.subject.meshInsulin-
dc.subject.meshMammary Glands, Animal-
dc.subject.meshProlactin-
dc.subject.meshRNA, Messenger-
dc.subject.meshSelenoproteins-
dc.subject.meshSodium Selenite-
dc.subject.meshTretinoin-
dc.titleRegulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.en
dc.typeArticleen
dc.identifier.journalJournal of Trace Elements in Medicine and Biologyen_GB

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