Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/10146/199930
Title:
Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells.
Authors:
Cronholm, Pontus; Midander, Klara; Karlsson, Hanna L.; Elihn, Karine; Wallinder, Inger Odnevall; Moller, Lennart
Abstract:
Abstract Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.
Citation:
Nanotoxicology 2011, 5 (2):269-281
Journal:
Nanotoxicology
Issue Date:
Jun-2011
URI:
http://hdl.handle.net/10146/199930
DOI:
10.3109/17435390.2010.536268
PubMed ID:
21117831
Additional Links:
http://informahealthcare.com/doi/abs/10.3109/17435390.2010.536268
Type:
Article
Language:
en
ISSN:
1743-5404
Sponsors:
This work was partly supported by the Swedish Research Council for Environment, Agricultural Sciences, and Spatial Planning (FORMAS), AForsk (Angpanneföreningen’s Foundation for Research and Development), and Environmental Cancer Risk, Nutrition, and Individual Susceptibility (ECNIS), a Network of Excellence operating within the European Union 6th Framework Program, Priority 5: “Food, Quality and Safety” (Contract No. 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorCronholm, Pontusen
dc.contributor.authorMidander, Klaraen
dc.contributor.authorKarlsson, Hanna L.en
dc.contributor.authorElihn, Karineen
dc.contributor.authorWallinder, Inger Odnevallen
dc.contributor.authorMoller, Lennarten
dc.date.accessioned2012-01-04T08:31:36Z-
dc.date.available2012-01-04T08:31:36Z-
dc.date.issued2011-06-
dc.identifier.citationNanotoxicology 2011, 5 (2):269-281en
dc.identifier.issn1743-5404-
dc.identifier.pmid21117831-
dc.identifier.doi10.3109/17435390.2010.536268-
dc.identifier.urihttp://hdl.handle.net/10146/199930-
dc.description.abstractAbstract Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.en
dc.description.sponsorshipThis work was partly supported by the Swedish Research Council for Environment, Agricultural Sciences, and Spatial Planning (FORMAS), AForsk (Angpanneföreningen’s Foundation for Research and Development), and Environmental Cancer Risk, Nutrition, and Individual Susceptibility (ECNIS), a Network of Excellence operating within the European Union 6th Framework Program, Priority 5: “Food, Quality and Safety” (Contract No. 513943).en
dc.language.isoenen
dc.relation.urlhttp://informahealthcare.com/doi/abs/10.3109/17435390.2010.536268en
dc.subjectNanoparticlesen
dc.subjectCopperen
dc.subjectToxicityen
dc.subjectAnimalsen
dc.subjectHumansen
dc.subjectEpithelial Cellsen
dc.subjectLungen
dc.subjectBlood Proteinsen
dc.subjectComet Assayen
dc.subjectNanotoxicologyen
dc.subject.meshAnimals-
dc.subject.meshBlood Proteins-
dc.subject.meshCell Line-
dc.subject.meshCell Survival-
dc.subject.meshComet Assay-
dc.subject.meshCopper-
dc.subject.meshEpithelial Cells-
dc.subject.meshHumans-
dc.subject.meshLung-
dc.subject.meshMaterials Testing-
dc.subject.meshMetal Nanoparticles-
dc.subject.meshParticle Size-
dc.subject.meshSonication-
dc.titleEffect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells.en
dc.typeArticleen
dc.identifier.journalNanotoxicologyen

Related articles on PubMed

All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.