Bulky DNA adducts in white blood cells: a pooled analysis of 3,600 subjects.

2.50
Hdl Handle:
http://hdl.handle.net/10146/193098
Title:
Bulky DNA adducts in white blood cells: a pooled analysis of 3,600 subjects.
Authors:
Ricceri, Fulvio; Godschalk, Roger W.; Peluso, Marco; Phillips, David H.; Agudo, Antonio; Georgiadis, Panagiotis; Loft, Steffen; Tjonneland, Anne; Raaschou-Nielsen, Ole; Palli, Domenico; Perera, Frederica; Vermeulen, Roel; Taioli, Emanuela; Sram, Radim J.; Munnia, Armelle; Rosa, Fabio; Allione, Alessandra; Matullo, Giuseppe; Vineis, Paolo
Abstract:
Bulky DNA adducts are markers of exposure to genotoxic aromatic compounds, which reflect the ability of an individual to metabolically activate carcinogens and to repair DNA damage. Polycyclic aromatic hydrocarbons (PAHs) represent a major class of carcinogens that are capable of forming such adducts. Factors that have been reported to be related to DNA adduct levels include smoking, diet, body mass index (BMI), genetic polymorphisms, the season of collection of biologic material, and air pollutants.; We pooled 11 studies (3,600 subjects) in which bulky DNA adducts were measured in human white blood cells with similar (32)P-postlabeling techniques and for which a similar set of variables was available, including individual data on age, gender, ethnicity, batch, smoking habits, BMI, and season of blood collection, and a limited set of gene variants.; Lowest DNA adduct levels (P = 0.006) were observed in the spring (median = 0.50 adducts per 10(8) nucleotides), followed by summer (0.64), autumn (0.70), and winter (0.85). The same pattern emerged in multivariate analysis but only among never smokers (P = 0.02). Adduct levels were significantly lower (P = 0.001) in northern Europe (the Netherlands and Denmark; mean = 0.60, median = 0.40) than in southern Europe (Italy, Spain, France, and Greece; mean = 0.79, median = 0.60).; In this large pooled analysis, we have found only weak associations between bulky DNA adducts and exposure variables. Seasonality (with higher adducts levels in winter) and air pollution may partly explain some of the interarea differences (north vs. south Europe), but most inter-area and interindividual variations in adduct levels still remain unexplained.; Our study describes the largest pooled analysis of bulky DNA adducts so far, showing that interindividual variation is still largely unexplained, though seasonality seems to play a role.
Citation:
Cancer Epidemiol. Biomarkers Prev. 2010, 19 (12):3174-3181
Journal:
Cancer Epidemiology, Biomarkers & Prevention
Issue Date:
Dec-2010
URI:
http://hdl.handle.net/10146/193098
DOI:
10.1158/1055-9965.EPI-10-0314
PubMed ID:
20921335
Additional Links:
http://cebp.aacrjournals.org/content/19/12/3174.long
Type:
Article
Language:
en
ISSN:
1538-7755
Sponsors:
This study was made possible by the ECNIS grant from the European Union (FOOD-CT-2005-513943) and by the Programma Integrato Oncologia, Italy (P.V.).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorRicceri, Fulvioen
dc.contributor.authorGodschalk, Roger W.en
dc.contributor.authorPeluso, Marcoen
dc.contributor.authorPhillips, David H.en
dc.contributor.authorAgudo, Antonioen
dc.contributor.authorGeorgiadis, Panagiotisen
dc.contributor.authorLoft, Steffenen
dc.contributor.authorTjonneland, Anneen
dc.contributor.authorRaaschou-Nielsen, Oleen
dc.contributor.authorPalli, Domenicoen
dc.contributor.authorPerera, Fredericaen
dc.contributor.authorVermeulen, Roelen
dc.contributor.authorTaioli, Emanuelaen
dc.contributor.authorSram, Radim J.en
dc.contributor.authorMunnia, Armelleen
dc.contributor.authorRosa, Fabioen
dc.contributor.authorAllione, Alessandraen
dc.contributor.authorMatullo, Giuseppeen
dc.contributor.authorVineis, Paoloen
dc.date.accessioned2011-12-05T12:24:15Z-
dc.date.available2011-12-05T12:24:15Z-
dc.date.issued2010-12-
dc.identifier.citationCancer Epidemiol. Biomarkers Prev. 2010, 19 (12):3174-3181en
dc.identifier.issn1538-7755-
dc.identifier.pmid20921335-
dc.identifier.doi10.1158/1055-9965.EPI-10-0314-
dc.identifier.urihttp://hdl.handle.net/10146/193098-
dc.description.abstractBulky DNA adducts are markers of exposure to genotoxic aromatic compounds, which reflect the ability of an individual to metabolically activate carcinogens and to repair DNA damage. Polycyclic aromatic hydrocarbons (PAHs) represent a major class of carcinogens that are capable of forming such adducts. Factors that have been reported to be related to DNA adduct levels include smoking, diet, body mass index (BMI), genetic polymorphisms, the season of collection of biologic material, and air pollutants.en
dc.description.abstractWe pooled 11 studies (3,600 subjects) in which bulky DNA adducts were measured in human white blood cells with similar (32)P-postlabeling techniques and for which a similar set of variables was available, including individual data on age, gender, ethnicity, batch, smoking habits, BMI, and season of blood collection, and a limited set of gene variants.en
dc.description.abstractLowest DNA adduct levels (P = 0.006) were observed in the spring (median = 0.50 adducts per 10(8) nucleotides), followed by summer (0.64), autumn (0.70), and winter (0.85). The same pattern emerged in multivariate analysis but only among never smokers (P = 0.02). Adduct levels were significantly lower (P = 0.001) in northern Europe (the Netherlands and Denmark; mean = 0.60, median = 0.40) than in southern Europe (Italy, Spain, France, and Greece; mean = 0.79, median = 0.60).en
dc.description.abstractIn this large pooled analysis, we have found only weak associations between bulky DNA adducts and exposure variables. Seasonality (with higher adducts levels in winter) and air pollution may partly explain some of the interarea differences (north vs. south Europe), but most inter-area and interindividual variations in adduct levels still remain unexplained.en
dc.description.abstractOur study describes the largest pooled analysis of bulky DNA adducts so far, showing that interindividual variation is still largely unexplained, though seasonality seems to play a role.en
dc.description.sponsorshipThis study was made possible by the ECNIS grant from the European Union (FOOD-CT-2005-513943) and by the Programma Integrato Oncologia, Italy (P.V.).en
dc.language.isoenen
dc.relation.urlhttp://cebp.aacrjournals.org/content/19/12/3174.longen
dc.subjectDNA Adductsen
dc.subjectHumansen
dc.subjectLeukocytesen
dc.subjectAir pollutionen
dc.subjectSeasonalityen
dc.subject.meshDNA Adducts-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshLeukocytes-
dc.subject.meshMale-
dc.subject.meshSeasons-
dc.titleBulky DNA adducts in white blood cells: a pooled analysis of 3,600 subjects.en
dc.typeArticleen
dc.identifier.journalCancer Epidemiology, Biomarkers & Preventionen

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