Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.

2.50
Hdl Handle:
http://hdl.handle.net/10146/114947
Title:
Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.
Authors:
Evans, Mark D.; Olinski, Ryszard; Loft, Steffen; Cooke, Marcus S.
Abstract:
Of the DNA-derived biomarkers of oxidative stress, urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is the most frequently measured. However, there is significant discrepancy between chromatographic and immunoassay approaches, and intratechnique agreement among all available chromatography-based assays and ELISAs is yet to be established. This is a significant obstacle to their use in large molecular epidemiological studies. To evaluate the accuracy of intra/intertechnique and interlaboratory measurements, samples of phosphate buffered saline and urine, spiked with different concentrations of 8-oxoG, together with a series of urine samples from healthy individuals were distributed to ESCULA members. All laboratories received identical samples, including 2 negative controls that contained no added 8-oxodG. Data were returned from 17 laboratories, representing 20 methods, broadly classified as mass spectrometric (MS), electrochemical detection (EC), or enzyme-linked immunosorbant assay (ELISA). Overall, there was good within-technique agreement, with the majority of laboratories' results lying within 1 sd of their consensus mean. However, ELISA showed more within-technique variation than did the chromatographic techniques and, for the urine samples, reported higher values. Bland-Altman plots revealed good agreement between MS and EC methods but concentration-dependent deviation for ELISA. All methods ranked urine samples according to concentration similarly. Creatinine levels are routinely used as a correction factor for urine concentration, and therefore we also conducted an interlaboratory comparison of methods for urinary creatinine determination, in which the vast majority of values lay within 1 sd of the consensus value, irrespective of the analysis procedure. This study reveals greater consensus than previously expected, although concern remains over ELISA.-ESCULA [European Standards Committee on Urinary (DNA) Lesion Analysis], Evans, M. D., Olinski, R., Loft, S., Cooke, M. S. Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.
Citation:
FASEB J. 2010, 24 (4):1249-60
Journal:
The FASEB Journal : official publication of the Federation of American Societies for Experimental Biology
Issue Date:
Apr-2010
URI:
http://hdl.handle.net/10146/114947
DOI:
10.1096/fj.09-147124
PubMed ID:
19966135
Additional Links:
http://www.fasebj.org/content/24/4/1249.long
Type:
Article
Language:
en
ISSN:
1530-6860
Sponsors:
Some of the authors of this paper are partners in, and this work was partly supported by, ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a Network of Excellence operating within the European Union 6th Framework Program, Priority 5: Food Quality and Safety (contract 513943)
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorEvans, Mark D.en
dc.contributor.authorOlinski, Ryszarden
dc.contributor.authorLoft, Steffenen
dc.contributor.authorCooke, Marcus S.en
dc.date.accessioned2010-11-08T12:03:41Z-
dc.date.available2010-11-08T12:03:41Z-
dc.date.issued2010-04-
dc.identifier.citationFASEB J. 2010, 24 (4):1249-60en
dc.identifier.issn1530-6860-
dc.identifier.pmid19966135-
dc.identifier.doi10.1096/fj.09-147124-
dc.identifier.urihttp://hdl.handle.net/10146/114947-
dc.description.abstractOf the DNA-derived biomarkers of oxidative stress, urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is the most frequently measured. However, there is significant discrepancy between chromatographic and immunoassay approaches, and intratechnique agreement among all available chromatography-based assays and ELISAs is yet to be established. This is a significant obstacle to their use in large molecular epidemiological studies. To evaluate the accuracy of intra/intertechnique and interlaboratory measurements, samples of phosphate buffered saline and urine, spiked with different concentrations of 8-oxoG, together with a series of urine samples from healthy individuals were distributed to ESCULA members. All laboratories received identical samples, including 2 negative controls that contained no added 8-oxodG. Data were returned from 17 laboratories, representing 20 methods, broadly classified as mass spectrometric (MS), electrochemical detection (EC), or enzyme-linked immunosorbant assay (ELISA). Overall, there was good within-technique agreement, with the majority of laboratories' results lying within 1 sd of their consensus mean. However, ELISA showed more within-technique variation than did the chromatographic techniques and, for the urine samples, reported higher values. Bland-Altman plots revealed good agreement between MS and EC methods but concentration-dependent deviation for ELISA. All methods ranked urine samples according to concentration similarly. Creatinine levels are routinely used as a correction factor for urine concentration, and therefore we also conducted an interlaboratory comparison of methods for urinary creatinine determination, in which the vast majority of values lay within 1 sd of the consensus value, irrespective of the analysis procedure. This study reveals greater consensus than previously expected, although concern remains over ELISA.-ESCULA [European Standards Committee on Urinary (DNA) Lesion Analysis], Evans, M. D., Olinski, R., Loft, S., Cooke, M. S. Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.en
dc.description.sponsorshipSome of the authors of this paper are partners in, and this work was partly supported by, ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a Network of Excellence operating within the European Union 6th Framework Program, Priority 5: Food Quality and Safety (contract 513943)en
dc.language.isoenen
dc.relation.urlhttp://www.fasebj.org/content/24/4/1249.longen
dc.subjectBiological Markersen
dc.subjectDeoxyguanosineen
dc.subjectCreatinineen
dc.subjectOxidative Stressen
dc.subjectHumansen
dc.subjectEnzyme-Linked Immunosorbent Assayen
dc.subjectElectrochemical Techniquesen
dc.subjectSensitivity and Specificityen
dc.subject.meshBiological Markers-
dc.subject.meshCreatinine-
dc.subject.meshDeoxyguanosine-
dc.subject.meshElectrochemical Techniques-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshEurope-
dc.subject.meshHumans-
dc.subject.meshOxidative Stress-
dc.subject.meshSensitivity and Specificity-
dc.titleToward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.en
dc.typeArticleen
dc.identifier.journalThe FASEB Journal : official publication of the Federation of American Societies for Experimental Biologyen

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