Reference genes for gene expression studies on non-small cell lung cancer.

2.50
Hdl Handle:
http://hdl.handle.net/10146/114772
Title:
Reference genes for gene expression studies on non-small cell lung cancer.
Authors:
Gresner, Peter ( 0000-0002-7008-396X ) ; Gromadzinska, Jolanta; Wasowicz, Wojciech ( 0000-0002-2991-9040 )
Abstract:
STUDY OBJECTIVE: The aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues. METHOD: Quantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients. RESULTS: The analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results. CONCLUSION: Although HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.
Citation:
Acta Biochim. Pol. 2009, 56 (2):307-316
Journal:
Acta Biochimica Polonica
Issue Date:
2009
URI:
http://hdl.handle.net/10146/114772
PubMed ID:
19543558
Additional Links:
http://www.actabp.pl/pdf/2_2009/307.pdf
Type:
Article
Language:
en
ISSN:
1734-154X
Sponsors:
This work was partially supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943). Dr. Peter Gresner is a fellow of EPITOK (Transfer of Knowledge in Molecular Biology and Epidemiology of Occupational and Environmental Cancer), a Marie Curie Action within the European Union 6th Framework Program (No. MTKDCT- 2004–509829).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGresner, Peteren
dc.contributor.authorGromadzinska, Jolantaen
dc.contributor.authorWasowicz, Wojciechen
dc.date.accessioned2010-11-05T11:39:39Zen
dc.date.available2010-11-05T11:39:39Zen
dc.date.issued2009en
dc.identifier.citationActa Biochim. Pol. 2009, 56 (2):307-316en
dc.identifier.issn1734-154Xen
dc.identifier.pmid19543558en
dc.identifier.urihttp://hdl.handle.net/10146/114772en
dc.description.abstractSTUDY OBJECTIVE: The aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues. METHOD: Quantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients. RESULTS: The analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results. CONCLUSION: Although HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.en
dc.description.sponsorshipThis work was partially supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943). Dr. Peter Gresner is a fellow of EPITOK (Transfer of Knowledge in Molecular Biology and Epidemiology of Occupational and Environmental Cancer), a Marie Curie Action within the European Union 6th Framework Program (No. MTKDCT- 2004–509829).en
dc.language.isoenen
dc.relation.urlhttp://www.actabp.pl/pdf/2_2009/307.pdfen
dc.subjectCarcinoma, Non-Small-Cell Lungen
dc.subjectLung Neoplasmsen
dc.subjectGene Expressionen
dc.subjectHumansen
dc.subjectRNA, Messengeren
dc.subjectPolymerase Chain Reactionen
dc.subject.meshAgeden
dc.subject.meshCarboxylesteraseen
dc.subject.meshCarcinoma, Non-Small-Cell Lungen
dc.subject.meshDNA Primersen
dc.subject.meshFemaleen
dc.subject.meshGene Expressionen
dc.subject.meshHumansen
dc.subject.meshHypoxanthine Phosphoribosyltransferaseen
dc.subject.meshLungen
dc.subject.meshLung Neoplasmsen
dc.subject.meshMaleen
dc.subject.meshMiddle Ageden
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshRNA, Messengeren
dc.subject.meshReference Standardsen
dc.subject.meshSoftwareen
dc.titleReference genes for gene expression studies on non-small cell lung cancer.en
dc.typeArticleen
dc.identifier.journalActa Biochimica Polonicaen

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