Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.

2.50
Hdl Handle:
http://hdl.handle.net/10146/114705
Title:
Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.
Authors:
Cooke, Marcus S.; Barregard, Lars; Mistry, Vilas; Potdar, Neelam; Rozalski, Rafal; Gackowski, Daniel; Siomek, Agnieszka; Foksinski, Marek; Svoboda, Peter; Kasai, Hiroshi; Konje, Justin C.; Sallsten, Gerd; Evans, Mark D.; Olinski, Ryszard
Abstract:
Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg(-1) creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.
Citation:
Biomarkers 2009, 14 (2):103-110
Journal:
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
Issue Date:
Mar-2009
URI:
http://hdl.handle.net/10146/114705
DOI:
10.1080/13547500802706012
PubMed ID:
19330588
Additional Links:
http://informahealthcare.com/doi/abs/10.1080/13547500802706012
Type:
Article
Language:
en
ISSN:
1366-5804
Sponsors:
This work was partly supported by ECNIS. M.S.C., M.D.E., R.R., D.G., A.S., M.F. and R.O. are partners of ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No. 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorCooke, Marcus S.en
dc.contributor.authorBarregard, Larsen
dc.contributor.authorMistry, Vilasen
dc.contributor.authorPotdar, Neelamen
dc.contributor.authorRozalski, Rafalen
dc.contributor.authorGackowski, Danielen
dc.contributor.authorSiomek, Agnieszkaen
dc.contributor.authorFoksinski, Mareken
dc.contributor.authorSvoboda, Peteren
dc.contributor.authorKasai, Hiroshien
dc.contributor.authorKonje, Justin C.en
dc.contributor.authorSallsten, Gerden
dc.contributor.authorEvans, Mark D.en
dc.contributor.authorOlinski, Ryszarden
dc.date.accessioned2010-11-04T11:26:16Z-
dc.date.available2010-11-04T11:26:16Z-
dc.date.issued2009-03-
dc.identifier.citationBiomarkers 2009, 14 (2):103-110en
dc.identifier.issn1366-5804-
dc.identifier.pmid19330588-
dc.identifier.doi10.1080/13547500802706012-
dc.identifier.urihttp://hdl.handle.net/10146/114705-
dc.description.abstractUrinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg(-1) creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.en
dc.description.sponsorshipThis work was partly supported by ECNIS. M.S.C., M.D.E., R.R., D.G., A.S., M.F. and R.O. are partners of ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No. 513943).en
dc.language.isoenen
dc.relation.urlhttp://informahealthcare.com/doi/abs/10.1080/13547500802706012en
dc.subjectDeoxyguanosineen
dc.subjectCreatinineen
dc.subjectElectrochemistryen
dc.subjectEnzyme-Linked Immunosorbent Assayen
dc.subjectGas Chromatography-Mass Spectrometryen
dc.subjectHumansen
dc.subjectLaboratoriesen
dc.subject.meshAdult-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshCreatinine-
dc.subject.meshDeoxyguanosine-
dc.subject.meshElectrochemistry-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshGas Chromatography-Mass Spectrometry-
dc.subject.meshHumans-
dc.subject.meshLaboratories-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.titleInterlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.en
dc.typeArticleen
dc.identifier.journalBiomarkers : biochemical indicators of exposure, response, and susceptibility to chemicalsen
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