Expression of selenoprotein-coding genes SEPP1, SEP15 and hGPX1 in non-small cell lung cancer.

2.50
Hdl Handle:
http://hdl.handle.net/10146/114441
Title:
Expression of selenoprotein-coding genes SEPP1, SEP15 and hGPX1 in non-small cell lung cancer.
Authors:
Gresner, Peter ( 0000-0002-7008-396X ) ; Gromadzinska, Jolanta; Jablonska, Ewa ( 0000-0002-3946-3964 ) ; Kaczmarski, Jacek; Wasowicz, Wojciech ( 0000-0002-2991-9040 )
Abstract:
Aim of the study was to investigate the mRNA expression level of selenoprotein P (SEPP1), 15-kDa selenoprotein (SEP15) and glutathione peroxidase 1 (hGPX1) in paired malignant and non-malignant tissue. To achieve this goal, the quantitative real-time PCR technique was utilized in paired tissue samples from 33 non-small cell lung cancer (NSCLC) patients. Simultaneously, the activity of glutathione peroxidases (GPX) and the level of thiobarbituric acid-reactive species (TBARS) in paired tissue specimens and the blood plasma selenium level was measured. We found significant down-regulation of SEPP1 expression level in tumorous lung tissue (2.732-fold; p<0.001). The expression of hGPX1 and SEP15 in tumorous tissue remained unchanged compared to healthy tissue. The level of TBARS in malignant tissue was significantly increased (p<0.005) and negatively correlated with SEPP1 expression level (R(S)=-0.3238; p<0.05). The activity of GPX in malignant tissue was significantly increased compared to the non-malignant one (p<0.005) and negatively correlated with the expression level of SEPP1. It seems possible, that the down-regulation of SEPP1 expression may lead to an increased oxidative stress possibly resulting in lung carcinogenesis. Increased activity of GPX in tumorous lung tissue seems to be a feedback mechanism.
Citation:
Lung Cancer 2009, 65 (1):34-40
Journal:
Lung Cancer (Amsterdam, Netherlands)
Issue Date:
Jul-2009
URI:
http://hdl.handle.net/10146/114441
DOI:
10.1016/j.lungcan.2008.10.023
PubMed ID:
19058871
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T9C-4V34RB8-4&_user=1843694&_coverDate=07%2F31%2F2009&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=409645ad1469a87a01970d4cb2acb925&searchtype=a
Type:
Article
Language:
en
ISSN:
1872-8332
Sponsors:
This work was partially supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No 513943). Dr Peter Gresner is a fellow of EPITOK (Transfer of Knowledge in Molecular Biology and Epidemiology of Occupational and Environmental Cancer), a Marie Curie Action within the European Union 6th Framework Program (No MTKDCT-2004-509829).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGresner, Peteren
dc.contributor.authorGromadzinska, Jolantaen
dc.contributor.authorJablonska, Ewaen
dc.contributor.authorKaczmarski, Jaceken
dc.contributor.authorWasowicz, Wojciechen
dc.date.accessioned2010-11-03T11:58:15Zen
dc.date.available2010-11-03T11:58:15Zen
dc.date.issued2009-07en
dc.identifier.citationLung Cancer 2009, 65 (1):34-40en
dc.identifier.issn1872-8332en
dc.identifier.pmid19058871en
dc.identifier.doi10.1016/j.lungcan.2008.10.023en
dc.identifier.urihttp://hdl.handle.net/10146/114441en
dc.description.abstractAim of the study was to investigate the mRNA expression level of selenoprotein P (SEPP1), 15-kDa selenoprotein (SEP15) and glutathione peroxidase 1 (hGPX1) in paired malignant and non-malignant tissue. To achieve this goal, the quantitative real-time PCR technique was utilized in paired tissue samples from 33 non-small cell lung cancer (NSCLC) patients. Simultaneously, the activity of glutathione peroxidases (GPX) and the level of thiobarbituric acid-reactive species (TBARS) in paired tissue specimens and the blood plasma selenium level was measured. We found significant down-regulation of SEPP1 expression level in tumorous lung tissue (2.732-fold; p<0.001). The expression of hGPX1 and SEP15 in tumorous tissue remained unchanged compared to healthy tissue. The level of TBARS in malignant tissue was significantly increased (p<0.005) and negatively correlated with SEPP1 expression level (R(S)=-0.3238; p<0.05). The activity of GPX in malignant tissue was significantly increased compared to the non-malignant one (p<0.005) and negatively correlated with the expression level of SEPP1. It seems possible, that the down-regulation of SEPP1 expression may lead to an increased oxidative stress possibly resulting in lung carcinogenesis. Increased activity of GPX in tumorous lung tissue seems to be a feedback mechanism.en
dc.description.sponsorshipThis work was partially supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No 513943). Dr Peter Gresner is a fellow of EPITOK (Transfer of Knowledge in Molecular Biology and Epidemiology of Occupational and Environmental Cancer), a Marie Curie Action within the European Union 6th Framework Program (No MTKDCT-2004-509829).en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T9C-4V34RB8-4&_user=1843694&_coverDate=07%2F31%2F2009&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=409645ad1469a87a01970d4cb2acb925&searchtype=aen
dc.subjectLung Neoplasmsen
dc.subjectSelenoproteinsen
dc.subjectGlutathione Peroxidaseen
dc.subjectRNA, Messengeren
dc.subjectHumansen
dc.subjectCase-Control Studiesen
dc.subject.meshAdulten
dc.subject.meshAgeden
dc.subject.meshAged, 80 and overen
dc.subject.meshCarcinoma, Non-Small-Cell Lungen
dc.subject.meshCase-Control Studiesen
dc.subject.meshFemaleen
dc.subject.meshGlutathione Peroxidaseen
dc.subject.meshHumansen
dc.subject.meshLung Neoplasmsen
dc.subject.meshMaleen
dc.subject.meshMiddle Ageden
dc.subject.meshRNA, Messengeren
dc.subject.meshSeleniumen
dc.subject.meshSelenoprotein Pen
dc.subject.meshSelenoproteinsen
dc.subject.meshThiobarbituric Acid Reactive Substancesen
dc.titleExpression of selenoprotein-coding genes SEPP1, SEP15 and hGPX1 in non-small cell lung cancer.en
dc.typeArticleen
dc.identifier.journalLung Cancer (Amsterdam, Netherlands)en

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