DNA adducts in normal colonic mucosa from healthy controls and patients with colon polyps and colorectal carcinomas.

2.50
Hdl Handle:
http://hdl.handle.net/10146/113890
Title:
DNA adducts in normal colonic mucosa from healthy controls and patients with colon polyps and colorectal carcinomas.
Authors:
Jonsson, Charlotte; Stal, Per; Sjoqvist, Urban; Akerlund, Jan-Erik; Lofberg, Robert; Moller, Lennart
Abstract:
Colon cancer is a multistage process where adenomatous polyps developing in a normal mucosa may further progress to neoplasia. DNA adducts are biomarkers linked to exposure to carcinogenic compounds, tumour formation and clinically observed cancer. Such DNA adducts have been detected in the mucosa of colon cancer patients. The aim of this study was to investigate whether there are differences in DNA adduct levels and patterns in mucosa from patients with colon cancer, polyps and non-cancerous controls and whether some DNA adducts could be markers for colon cancer development. Human colonic biopsies were collected from healthy controls (n = 10), polyp patients (n = 22) (from both normal and polyp tissue) and colon cancer patients (n = 32) (from both tumour tissue and adjacent normal mucosa). In 150 tissues specimens (when small amount of tissue, the same type of tissues were pooled from each patient), DNA adduct levels and patterns were analysed by the (32)P-high-performance liquid chromatography method. There were no significant difference in the total levels of DNA adducts between any of the groups. Levels of two single DNA adducts were decreased in mucosa adjacent to tumours as compared to mucosa from healthy controls. One DNA adduct was found only in tumour tissue and adjacent mucosa from the colon cancer patients. A food derived, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-related DNA adduct was detected in 106 of the 150 tissues analysed, but in similar levels in tissues from controls, polyp patients or cancer patients. In conclusion, three individual DNA adducts may be interesting candidates for further evaluation of their possible role as biomarkers in human carcinogenesis. Furthermore, a food-derived PhIP-related adduct contributes to the general DNA adduct pattern in most individuals, indicating a minor role of this adduct in human colon carcinogenesis.
Citation:
Mutagenesis 2010, 25 (5):499-504
Journal:
Mutagenesis
Issue Date:
Sep-2010
URI:
http://hdl.handle.net/10146/113890
DOI:
10.1093/mutage/geq033
PubMed ID:
20551081
Additional Links:
http://mutage.oxfordjournals.org/content/25/5/499.long
Type:
Article
Language:
en
ISSN:
1464-3804
Sponsors:
Swedish Environmental Protection Agency; MISTRA Foundation; Swedish Society of Medicine (Bengt Ihres Fund); Environmental Cancer Risk, Nutrition and Individual Susceptibility, a network of excellence operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorJonsson, Charlotteen
dc.contributor.authorStal, Peren
dc.contributor.authorSjoqvist, Urbanen
dc.contributor.authorAkerlund, Jan-Eriken
dc.contributor.authorLofberg, Roberten
dc.contributor.authorMoller, Lennarten
dc.date.accessioned2010-10-27T12:48:30Z-
dc.date.available2010-10-27T12:48:30Z-
dc.date.issued2010-09-
dc.identifier.citationMutagenesis 2010, 25 (5):499-504en
dc.identifier.issn1464-3804-
dc.identifier.pmid20551081-
dc.identifier.doi10.1093/mutage/geq033-
dc.identifier.urihttp://hdl.handle.net/10146/113890-
dc.description.abstractColon cancer is a multistage process where adenomatous polyps developing in a normal mucosa may further progress to neoplasia. DNA adducts are biomarkers linked to exposure to carcinogenic compounds, tumour formation and clinically observed cancer. Such DNA adducts have been detected in the mucosa of colon cancer patients. The aim of this study was to investigate whether there are differences in DNA adduct levels and patterns in mucosa from patients with colon cancer, polyps and non-cancerous controls and whether some DNA adducts could be markers for colon cancer development. Human colonic biopsies were collected from healthy controls (n = 10), polyp patients (n = 22) (from both normal and polyp tissue) and colon cancer patients (n = 32) (from both tumour tissue and adjacent normal mucosa). In 150 tissues specimens (when small amount of tissue, the same type of tissues were pooled from each patient), DNA adduct levels and patterns were analysed by the (32)P-high-performance liquid chromatography method. There were no significant difference in the total levels of DNA adducts between any of the groups. Levels of two single DNA adducts were decreased in mucosa adjacent to tumours as compared to mucosa from healthy controls. One DNA adduct was found only in tumour tissue and adjacent mucosa from the colon cancer patients. A food derived, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-related DNA adduct was detected in 106 of the 150 tissues analysed, but in similar levels in tissues from controls, polyp patients or cancer patients. In conclusion, three individual DNA adducts may be interesting candidates for further evaluation of their possible role as biomarkers in human carcinogenesis. Furthermore, a food-derived PhIP-related adduct contributes to the general DNA adduct pattern in most individuals, indicating a minor role of this adduct in human colon carcinogenesis.en
dc.description.sponsorshipSwedish Environmental Protection Agency; MISTRA Foundation; Swedish Society of Medicine (Bengt Ihres Fund); Environmental Cancer Risk, Nutrition and Individual Susceptibility, a network of excellence operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No 513943).en
dc.language.isoenen
dc.relation.urlhttp://mutage.oxfordjournals.org/content/25/5/499.longen
dc.subjectColon canceren
dc.subjectBiomarkersen
dc.subjectDNA adductsen
dc.subjectMucosaen
dc.subject.meshCase-Control Studiesen
dc.subject.meshChromatography, High Pressure Liquiden
dc.subject.meshColonic Polypsen
dc.subject.meshColorectal Neoplasmsen
dc.subject.meshDNA Adductsen
dc.subject.meshHealthen
dc.subject.meshHumansen
dc.subject.meshImidazolesen
dc.subject.meshIntestinal Mucosaen
dc.titleDNA adducts in normal colonic mucosa from healthy controls and patients with colon polyps and colorectal carcinomas.en
dc.typeArticleen
dc.identifier.journalMutagenesisen

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