Assessment and reduction of comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group.

2.50
Hdl Handle:
http://hdl.handle.net/10146/113553
Title:
Assessment and reduction of comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group.
Authors:
Moller, Peter; Moller, Lennart; Godschalk, Roger W. L.; Jones, George D. D.
Abstract:
The alkaline single cell gel electrophoresis (comet) assay has become a widely used method for the detection of DNA damage and repair in cells and tissues. Still, it has been difficult to compare results from different investigators because of differences in assay conditions and because the data are reported in different units. The European Comet Assay Validation Group (ECVAG) was established for the purpose of validation of the comet assay with respect to measures of DNA damage formation and its repair. The results from this inter-laboratory validation trail showed a large variation in measured level of DNA damage and formamidopyrimidine DNA glycosylase-sensitive sites but the laboratories could detect concentration-dependent relationships in coded samples. Standardization of the results with reference standards decreased the inter-laboratory variation. The ECVAG trail indicates substantial reliability for the measurement of DNA damage by the comet assay but there is still a need for further validation to reduce both assay and inter-laboratory variation.
Citation:
Mutagenesis 2010, 25 (2):109-111
Journal:
Mutagenesis
Issue Date:
Mar-2010
URI:
http://hdl.handle.net/10146/113553
DOI:
10.1093/mutage/gep067
PubMed ID:
20064897
Additional Links:
http://mutage.oxfordjournals.org/content/25/2/109.long
Type:
Article
Language:
en
ISSN:
1464-3804
Sponsors:
ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorMoller, Peteren
dc.contributor.authorMoller, Lennarten
dc.contributor.authorGodschalk, Roger W. L.en
dc.contributor.authorJones, George D. D.en
dc.date.accessioned2010-10-20T13:27:15Z-
dc.date.available2010-10-20T13:27:15Z-
dc.date.issued2010-03-
dc.identifier.citationMutagenesis 2010, 25 (2):109-111en
dc.identifier.issn1464-3804-
dc.identifier.pmid20064897-
dc.identifier.doi10.1093/mutage/gep067-
dc.identifier.urihttp://hdl.handle.net/10146/113553-
dc.description.abstractThe alkaline single cell gel electrophoresis (comet) assay has become a widely used method for the detection of DNA damage and repair in cells and tissues. Still, it has been difficult to compare results from different investigators because of differences in assay conditions and because the data are reported in different units. The European Comet Assay Validation Group (ECVAG) was established for the purpose of validation of the comet assay with respect to measures of DNA damage formation and its repair. The results from this inter-laboratory validation trail showed a large variation in measured level of DNA damage and formamidopyrimidine DNA glycosylase-sensitive sites but the laboratories could detect concentration-dependent relationships in coded samples. Standardization of the results with reference standards decreased the inter-laboratory variation. The ECVAG trail indicates substantial reliability for the measurement of DNA damage by the comet assay but there is still a need for further validation to reduce both assay and inter-laboratory variation.en
dc.description.sponsorshipECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety’ (Contract No 513943).en
dc.language.isoenen
dc.relation.urlhttp://mutage.oxfordjournals.org/content/25/2/109.longen
dc.subjectDNA Damageen
dc.subjectDNA-Formamidopyrimidine Glycosylaseen
dc.subjectComet Assayen
dc.subjectHumansen
dc.subjectHela Cellsen
dc.subjectLaboratoriesen
dc.subjectReference Standardsen
dc.subjectEuropeen
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshDNA-Formamidopyrimidine Glycosylase-
dc.subject.meshEurope-
dc.subject.meshFemale-
dc.subject.meshHela Cells-
dc.subject.meshHumans-
dc.subject.meshLaboratories-
dc.subject.meshMale-
dc.subject.meshObserver Variation-
dc.subject.meshReference Standards-
dc.titleAssessment and reduction of comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group.en
dc.typeArticleen
dc.identifier.journalMutagenesisen

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